豚鼠胆囊胆固醇结石形成过程中胆囊Cajal样间质细胞及动力的变化  

Changes of interstitial Cajal-like cells and gallbladder motility during the formation of cholesterol stones in guinea pig gallbladder

作　　者：范莹[1] 吴硕东[1] 殷振华[1] 付倍蓓 

机构地区：[1]中国医科大学附属盛京医院胆道、血管外科,沈阳110004 [2]小儿血液内科

出　　处：《中华实验外科杂志》2012年第11期2131-2133,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81000183）

摘　　要：目的观察豚鼠胆囊胆固醇结石形成过程中胆囊组织Cajal样间质细胞（ICLCs）及胆囊动力的变化。方法将100只豚鼠随机分为实验组与对照组，每组50只，实验组给予致石饲料（胆固醇含量2％），对照组给予正常颗粒饲料，8周造模结束后，利用胆囊全层铺片免疫荧光化学染色及激光共聚焦显微镜观察各组ICLCs数量的变化，利用透射电镜观察各组胆囊组织中ICLCs的超微结构特征，利用在体胆囊收缩素（CCK-8）激发实验评价胆囊动力的变化。结果对照组豚鼠胆囊ICLCs平均阳性面积为（18．37±0．64）％，实验组为（2．47±0．28）％，较对照组明显降低（t=21．122，P〈0．01）；对照组豚鼠胆囊胆汁时间．流量曲线下面积为（31．58±2．26）μl，实验组为（15．02±1．98）μl，较对照组明显降低（t=8．586，P〈0．01）。结论饮食诱导的豚鼠胆囊胆固醇结石形成过程中，胆囊ICLCs明显减少，同时胆囊动力受损，对外源性CCK反应下降。胆囊动力受损可能与ICLCs减少有关。Objective To analyze the changes of interstitial Cajal-like cells （ICLCs） and gall- bladder motility during the process of gallbladder cholesterol stone formation in the guinea pig model. Methods 100 guinea pigs were divided into two groups of 50 each. The animals in the experimental group were fed on a high cholesterol diet （ cholesterol 2% ） , while those in the control group fed on a normal diet. The guinea pigs were sacrificed at the 8th week. The morphological and distribution characteristics of ICLCs were observed by using immunohistochemistry. Ultrastructure of ICLCs was observed under a transmission electron microscope. Gallbaldder motility was assessed by cholecystokinin （GCK）-8 stimulation test in vivo. Results The average positive area of ICLCs was decreased obviously in the experimental group as compared with the control group [ （ 18.37±0. 64 ） % vs （ 2.47 ± 0.28 ） %, t = 21. 122, P 〈 0. 01 ]. The area under the time-flow curve of the gallbladder juice was decreased significantly in the experimental group as compared with the control group [ （ 31.58± 2. 26） μl vs （ 15.02± 1.98） μl, t = 8. 586, P 〈 0. 01 ]. Conclusion Decreased number of ICLCs and impaired gallbladder motility are present in guinea pigs fed on a lithogenous diet, and this may have a role in the pathogenesis of cholesterol gallstone formation.

关 键 词：胆固醇结石 胆囊 间质细胞 胆囊收缩素 

分 类 号：R575.62[医药卫生—消化系统]