红花对肝星状细胞基质分解素-1及其抑制因子基因表达的调节  

Regulation of carthamus tinctorius on gene expression of stromelysin-1 and tissue inhibitor of metal- loproteinase-1 in HSC-T6 cells

作　　者：王爱民[1] 阴桢宏[2] 张莉[1] 马雪梅[2] 耿焱[1] 王宝恩[2] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2012年第11期2165-2166,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察红花对HSC-T6细胞基质分解素-1（MMP3）及其抑制因子基因表达的影响。方法以不同浓度的红花（1．0、0．5g／L）作用于培养的肝星状细胞株HSC-T6细胞48h，收集细胞，提取总RNA；用逆转录-聚合酶链反应（RT-PCR）方法测定MMP3及金属蛋白酶组织抑制因子-1（TIMP-1）的基因表达水平。结果MMP3基因表达水平在红花1．0、0．5g／L两组分别为2．80±0．36、2．52±0．32，而空白对照组为2．13±0．30。TIMP-1基因表达水平在红花1．0、0．5g／L两组分别为1．66±0．23、2．04±0．30，而空白对照组为4．03±0．56。结论红花可增强HSC-T6细胞MMP3的基因表达，并显著抑制HSC-T6细胞TIMP-1的基因表达，且与剂量有关。Objective To investigate the effects of carthamus tinctorius on gene expression of stromelysin-1 （MMP3） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in HSC-T6 cells. Methods HSC-T6 cells were cultured in the medium containing different concentrations of carthamus tinctorius （ 1.0, and 0. 5 g/L） and collected after incubation for 48 h. Total RNA of HSC-T6 cells was isolated and gene expression levels of MMP3 and TIMP-1 were measured by using reverse transcription polymerase chain reaction （RT-PCR）. Results MMP3 gene expression levels in 1.0 and 0.5 g/L carthamus tinctorius groups were 2. 80 ±0. 36 and 2. 52 ±0. 32 respectively, and that in control group was 2. 13 ±0. 30. TIMP-1 gene expression level in 1.0 and 0. 5 g/L carthamus tinctorius groups were 1.66 ± 0. 23 and 2. 04 ± 0. 30 respectively, and that in control group was 4. 03 ± 0. 56. Conclusion Carthamus tinctori＇us may enhance the expression of MMP3, but significantly inhibit TIMP-1 gene expression of HSC-T6 cells in a concentration-dependent manner.

关 键 词：肝星状细胞 红花 基质分解素-1 金属蛋白酶组织抑制因子-1 基因表达 

分 类 号：R575.2[医药卫生—消化系统]