丹参对肝星状细胞基质分解素-1及其抑制因子基因表达的影响  

Effects of salvia miltiorrhiza on gene expression of stromelysin-1 and tissue inhibitor of metallopro- teinase-1 in HSC-T6 cells

作　　者：王爱民[1] 阴桢宏[2] 魏艳荣[1] 马雪梅[2] 李洵[1] 王宝恩[2] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2012年第11期2173-2174,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察丹参对HSC-T6细胞基质分解素-1（MMP3）及其抑制因子基因表达的影响。方法以不同浓度的丹参（0．8、0．4g／L）作用于培养的肝星状细胞株HSC-T6细胞48h，收集细胞，提取总RNA；用逆转录-聚合酶链反应（RT-PCR）方法测定MMP3及基质金属蛋白酶组织抑制因子-1（TIMP-1）的基因表达水平。结果MMP3基因表达水平在丹参0．8、0．4g／L两组分别为2．03±0．32、2．19±0．26，而空白对照组为2．13±0．30。TIMP-1基因表达水平在丹参0．8g／L、0．4g／L两组分别为2．11±0．32、3．12±0．46，而空白对照组为4．03±0．56。结论丹参可明显抑制HSC-T6细胞TIMP-1的基因表达，且与剂量有关；但对MMP3的基因表达无明显影响。Objective To investigate the effects of salvia mihiorrhiza on gene expression of stromelysin-1 （ MMP3 ） and tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） in HSC-T6 cells. Methods HSC-T6 cells were cultured in the medium containing different concentrations of salvia miltiorrhiza （0. 8 and 0.4 g/L） and collected after for 48 h. Total RNA of HSC-T6 cells was isolated and gene expression levels of MMP3 and T!MP-2 were measured by using reverse transcription polymerase chain reaction. Re- sults MMP3 gene expression levels in 0. 8 and 0.4 g/L salvia miltiorrhiza groups were 2. 03 ± 0. 32 and 2. 19±0.26 respectively, and those in control group was 2.13 ±0. 30. TIMP-1 gene expression levels in 0. 8 and 0.4 g/L salvia miltiorrhiza groups were 2. 11 ±0. 32 and3.12 ±0. 46 respectively, and those in control group was 4. 03 ±0. 56. Conclusion Salvia mihiorrhiza may significantly inhibit TIMP-1 gene expression in HSC-T6 cells in a concentration-dependent manner. Salvia miltiorrhiza has no effeet on MMP3 gene expression.

关 键 词：肝星状细胞 丹参 基质金属蛋白酶组织抑制因子-1 基因表达 

分 类 号：R575.2[医药卫生—消化系统]