Yes相关蛋白在胃腺癌中的表达及其下调对胃癌细胞增殖和转移的影响  

Expression of Yes-associated protein in gastric adenocarcinoma and effects of Yes-associated proteinknockdown on proliferation and metastasis of gastric cancer cells

作　　者：张靖[1] 许志朋[1] 朱金水[1] 周洲[1] 陈维雄[1] 陈尼维[1] 

机构地区：[1]上海交通大学附属第六人民医院消化内科,200233

出　　处：《中华实验外科杂志》2012年第11期2175-2177,共3页Chinese Journal of Experimental Surgery

基　　金：上海市科委资助项目（10140902500）;上海交通大学医学院博士创新基金资助项目（BXJ201234）;

摘　　要：目的检测Yes相关蛋白（YAP）在人胃腺癌、胃腺瘤及正常胃黏膜组织中的表达，探讨YAP对胃癌细胞增殖和转移的作用及其分子机制。方法免疫组织化学法和实时定量聚合酶链反应（Real-timePCR）检测YAP在78例人胃腺癌、39例人胃腺瘤及46例正常人胃黏膜组织的表达。利用建立慢病毒小发夹RNA（shRNA）靶向YAP基因的胃癌SGC-7901细胞株，Westernblot法检测增殖细胞核抗原（PCNA）和基质金属蛋白酶-2（MMP-2）的蛋白表达水平。噻唑蓝（MTT）比色法和Transwell试验检测YAP下调对胃癌细胞增殖和转移的影响。结果YAP在69．23％的胃腺癌组织中高表达，与正常胃黏膜（0．33±0．15）和胃腺瘤（0．42±0．20）比较，YAPmRNA在胃腺癌（1．74±0．46）的表达水平明显增高（P〈0．001）。体外实验显示，YAP下调减少PCNA和MMP-2蛋白的表达，并抑制胃癌细胞增殖活性和转移能力。结论YAP在人胃腺癌组织中高表达，并且YAP下调抑制胃癌细胞的增殖和转移。Objective To examine the expression of Yes-associated protein （YAP） in human gas- tric adenocarcinomas, gastric adenomas and normal gastric mucosa tissues, and further explore the effects and molecular mechanisms of YAP on proliferation and metastasis of gastric cancer cells. Methods The expression of YAP in 78 cases of gastric adenocarcinomas, 39 cases of gastric adenomas and 46 cases of normal gastric mucosa tissues was detected by using immunohistochemistry and real-time polymerase chain reaction （PCR）. In vitro human gastric cancer SGC-7901 cells were stably transfected with lentivirus-medi- ated YAP small hairpin RNA （ shRNA）, and the expression levels of proliferating cell nuclear antigen （PCNA） and metalloproteinase-2 （MMP-2） were detected by using Western blotting. The proliferative ac- tivity and metastatic capability of gastric cancer cells were evaluated by methyl thiazol tetrazolium （MTF） and Transwell assays. Results The expression of YAP was observed in 69. 23% gastric adenocarcinoma tissues, and its mRNA expression level in gastric adenocarcinoma （1.74 ± 0. 46） was significantly in- creased as .compared with that in normal gastric mucosa （0. 33 ±0. 15 ） and gastric adenoma （0.42±0. 20）. Knockdown of YAP by shRNA could down-regulate the expression of PCNA and MMP-2, and inhibit the proliferation and metastasis of gastric cancer cells. Conclusion YAP is strongly expressed in gas- tric adenocarcinoma, and knockdown of YAP inhibits proliferation and metastasis of gastric cancer cells.

关 键 词：胃癌 Yes相关蛋白 小发夹RNA 增殖 转移 

分 类 号：R735.2[医药卫生—肿瘤]