吗啡和芬太尼对人胃癌MGC-803细胞迁移能力的影响  

Effects of morphine or fentanyl on the migration ability of human gastric carcinoma MGC-803 cells

作　　者：管恩健[1] 覃怡[1] 利莉[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁530021

出　　处：《中华实验外科杂志》2012年第11期2178-2179,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81160289）;广西科学研究与技术开发计划项目（桂科攻1140003A.12）;广西教育厅科研资助项目（201012MS036）;广西研究生教育创新计划项目（2叭0105981002M199）

摘　　要：目的观察吗啡、芬太尼对人胃癌MGC-803细胞迁移能力的影响。方法将含0．1、10．0、1000．0μmol／L吗啡及0．0001、0．0100、1．0000μmol／L芬太尼的细胞培养液，分别与胃癌MGC-803细胞一同孵育48h，同时设空白对照组。应用划痕试验检测细胞迁移能力，应用半定量逆转录．聚合酶链反应（RT-PCR）和Westernblot技术检测胃癌MGC-803细胞中磷酸酶基因（PTEN）mRNA和蛋白的表达。结果划痕损伤48h后，各吗啡组及各芬太尼组细胞的愈合率均低于对照组，细胞迁移能力下降（P〈0．05）；RT-PCR和Westernblot结果显示，吗啡或芬太尼均使胃癌MGC-803细胞PTENmRNA和蛋白表达增高（P〈0．05）。结论吗啡和芬太尼可通过促进PTEN的表达而使胃癌MGC-803细胞的迁移能力降低。Objective To explore the effects of morphine or fentanly on the migration ability of human gastric carcinoma MGC-803 cells. Methods MGC-803 cells were incubated with 0. 1, 10.0 and 1000.0 μmol/L morphine, 0. 0001, 0. 0100 or 1. 0000 p, mol/L fentanyl respectively. The wound healing assay was used to detect the migration activity of MGC-803 cells. The expression of PTEN mRNA and pro- tein was detected by using semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and Western blotting. Results The healing rate in 0. 1 p.mol/L morphine group, 10. 0 μmol/L morphine group, 1000. 0μmol/L morphine group, 0. 0001 p.mol/L fentanyl group, 0. 0100 μmol/L fentanyl group or 1. 0000 μmol/L fentanyl group was 29. 17%, 20. 66%, 18. 87% , 15.51% , 24.98% or 22. 77%, lower than that of the control group, respectively. PTEN mRNA and protein were upregulated in morphine or fentanly groups. Conclusion Morphine and fentanly may suppress the migration activity of human gas- tric carcinoma MGC-803 cells by PTEN upregulation in vitro.

关 键 词：吗啡 芬太尼 胃癌 迁移 

分 类 号：R735.2[医药卫生—肿瘤]