白花蛇舌草诱导人胶质瘤细胞凋亡的作用及机制  

Mechanism of apoptosis induction by Hedyotic diffusa on human glioma cells

作　　者：张焱[1] 祝新根[1] 程祖珏[1] 沈晓黎[1] 郭华[1] 毛国华[1] 朱健明[1] 胡尚伟[1] 

机构地区：[1]南昌大学第二附属医院神经外科,330006

出　　处：《中华实验外科杂志》2012年第11期2222-2224,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨白花蛇舌草诱导胶质瘤U87细胞株凋亡的作用及机制。方法采用不同浓度的白花蛇舌草含药培养基（0、100、200ml／L）处理U87细胞48h，流式细胞仪检测对照组与实验组细胞的凋亡率及线粒体膜电位差异，Western blot法检测两组细胞中抗凋亡基因[B细胞淋巴瘤／白血病-2（bcl-2）]和促凋亡基因（bax）的表达水平改变。结果经100、200ml／L白花蛇舌草处理的U87细胞凋亡率明显增加，分别为（17．31±1．36）％、（41．23±0．72）％，明显高于对照组的（8．11±1．71）％（P〈0．01），呈显著的剂量依赖性；经JC-1染色法处理后，呈绿色荧光的U87细胞比率随含药培养基浓度升高而增多，分别为（11．90±0．20）％、（34．17±2．79）％，明显高于对照组的（5．53±1．20）％（P〈0．05），提示其线粒体膜电位受药物影响而逐步递减；白花蛇舌草能显著上调促凋亡蛋白bax基因的表达并降低bcl-2／bax比率。结论白花蛇舌草通过线粒体依赖性途径诱导人胶质瘤U87细胞的凋亡。Objective To investigate the possible mechanism of apoptosis induction by hedyotic diffusa （HD） on human glioma U87 cells. Methods U87 cells were cultured for 24 h in the medium con- taining HD with different concentrations （0, 100, 200 mE/L）. The apoptosis of U87 cells and collapse of mitoehondrial membrane potential were examined by flow cytometry. The protein expression levels of bel-2 and bax were detected by using immunoblot. Results The apoptosis rate in 100 and 200 ml/L HD groups was （ 17.31 ±1.36）% and （41.23 ±0. 72）% respectively, significantly higher than that in 0 ml/L HD group （P 〈 0. 01 ）. The JC-1-green-bright rate of cells treated with 100 and 200 mE/L HD was （11.90 ± 0.20） % and （ 34. 17 ± 2. 79 ） % respectively, significantly higher than untreated U87 cells （ P 〈 0.05 ）. The protein expression level of bax was increased and bcl-2-to-bax ratio was reduced dose-dependently. Conclusion HD induced apoptosis of U87 cells via mitoehondrion pathway.

关 键 词：白花蛇舌草 脑胶质瘤 脱噬作用 线粒体 

分 类 号：R739.41[医药卫生—肿瘤]