酶解栀子苷制备苷元的正交试验研究  

Orthogonal test for preparing genipin with enzymolyzed geniposide

作　　者：钮小松[1,2] 孟兆青[1,2,3] 程宁波[1,2] 朱经艳[1,2] 丁岗[1,2,3] 毕宇安[1,2,3] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏省企业院士工作站,江苏连云港222001

出　　处：《中国中药杂志》2012年第21期3236-3239,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2010CB735604)

摘　　要：目的:正交试验优化酶解栀子苷制备苷元工艺。方法:以栀子苷元浓度为评价指标,以加酶量、酶解液pH、酶解时间、酶解温度为考察因素,通过正交试验选取最佳酶解工艺。结果:最佳水解条件如下:选用一定纯度的栀子苷粗品,在摇床转速100 r·min-1,栀子苷质量浓度40 g·L-1下,按酶苷质量比1∶1加β-葡萄糖苷酶,酶解液pH 4.5,酶解20 h,酶解温度50℃,栀子苷元转化率可达85.8%。结论:该工艺稳定、可行,可为栀子苷酶解制备苷元提供一定理论依据。Objective： To optimize the process for preparing genipin with enzymolyzed geniposide by an orthogonal experiment. Method ： The optimal enzymolysis process was selected by an orthogonal experiment, with the concentration of geniposide as the index as well as enzyme quantity, pH of enzymolysis solution, enzymolysis time and enzymolysis temperature as considerations. Result： The optimal hydrolysis conditions were as follow： rough genipin samples at the concentration of 40 g·L ^-1were selected and shook on a ta- ble concentrator at a speed of 100 r · min^-1 , added with β-glucosidase-geniposide 1：1 （weight proportion）, with pH of enzymolysis solution at 4.5, hydrolyzation temperature at 50 ℃ , the conversion rate of genipin could reach 85, 8%. Conclusion： The process is so stable and feasible that it can provide theoretical basis for the preparation of genipin with enzymolyzed geniposide.

关 键 词：栀子苷 苷元 酶水解 正交试验 

分 类 号：R286.0[医药卫生—中药学]