向日葵染色体Giemsa C-分带研究  

Giemsa C-banding Patterns Analysis of Sunflower Chromosomes

作　　者：高猛[1,2] 安玉麟[2] 孙瑞芬[2] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019 [2]内蒙古农牧业科学院生物技术中心,呼和浩特010031

出　　处：《植物遗传资源学报》2012年第6期1078-1081,共4页Journal of Plant Genetic Resources

基　　金：国家现代向日葵产业技术体系项目(nycytx-z1)

摘　　要：采用HKG(HCl-KOH-Giemsa)法对内葵杂3号三交种染色体进行了C-分带研究和分析。结果表明:每条染色体至少都有一条C-分带,染色体组共有62条C-分带,以中间带和着丝点带为主,中间带主要分布在染色体短臂上;C-分带强弱差异明显,其中46条强带,16条弱带。Giemsa C-分带带型公式为:2n=2x=34=8I++3T++5I+I+T++4C+2CI+4CI++3CI++I+T++CT++2CT+。每条染色体都显示出显著的带纹特征,因此,利用Giemsa C-分带方法可以将向日葵的每条染色体区分开。In this paper,the HKG （HC1-KOH-Giemsa）method studied and analysised the C-banding of chro- mosomes in triple cross of Inner Mongolia Hibrid oil sunflower 3. The results showed that every chromosome had at least one C-banding and there were 62 C-bandings on the chromosome pairs, most of which were eentromeric and in- terbands,and the interbands were mainly distributed on the short arms;C-banding intensity were different ,46 were strong and 16 were weak. The C-bandings formula was 2n = 2x = 34 -- 8I ＋ ＋ 3T ＋ 5I ＋ I T ＋ ＋ 4C ＋ 2CI ＋ 4CI ＋ ＋ 3CI＋ ＋I＋T＋ ＋ CT＋ ＋2CT＋. Each chromosome had its own unique band pattern distinguishable from all the oth- ers,so each chromosome of sunflower can be distinguished by Giemsa C-banding.

关 键 词：向日葵(Helianthus annus L ) 染色体 GIEMSA C-分带 

分 类 号：S565.5[农业科学—作物学]