溴阻燃剂对肝细胞的毒性  

Hepatotoxicity of Brominated Flame Retardants

作　　者：那广水[1] 孙丽娟[2] 张雯雯[2] 王惠国[2] 

机构地区：[1]国家海洋环境监测中心国家海洋局近岸海域生态环境重点实验室,大连116023 [2]大连大学生命科学与技术学院,大连116622

出　　处：《生态毒理学报》2012年第5期570-573,共4页Asian Journal of Ecotoxicology

基　　金：海洋公益性科研专项(201105013)

摘　　要：为评价四溴联苯醚(2,2,4,4-tetrabromodipheny lether,PBDE-47)、五溴联苯醚(2,2',4,4',5-pentabromodiphenylether,PBDE-99)、九溴联苯醚(polybrominated dipheny lethers206,PBDE-206)、六溴环十二烷(hexabromocyclododecane,HBCD)、四溴双酚A(tetrabromobisphenol A,TBBPA)对人肝细胞(LO2)的体外细胞毒性。将5种溴阻燃剂与人肝细胞LO2共同培养,采用四甲基偶氮噻唑蓝比色法(MTT法)检测5种溴阻燃剂对LO2细胞的相对增殖率,并按照GB/T16886.5-2003/ISO10993-5:1999标准评价5种溴阻燃剂对LO2的细胞毒性。体外实验表明,5种溴阻燃剂对LO2细胞均具有抑制作用,其中PBDE-47、PBDE-99和HBCD呈现剂量依赖关系。To test the toxic effects of 2, 2, 4, 4-tetrabromodiphenyl ether （PBDE-47）, 2,2＇A,4＇,5-pentabromodi- phenyl ether （PBDE-99）, polybrominated diphenyl ethers 206 （PBDE-206）, hexabromocyclododecane （HBCD） and tetrabromobisphenol A （TBBPA） on normal human hepatocytes （LO2） in vitro, the human liver cell line LO2 was co-cultured with these five brominated flame retardants respectively. The inhibition rate of LO2cells was detected by MTT （3-（4,5-Dimethylthia zol-2-yl）-2,5-diphenyltetrazolium bromide） assay, and the cytotoxici- ty was evaluated in accordance with the criterion of GB/T16886.5-2003/ISO10993-5： 1999. As a result, these five brominated flame retardants showed inhibiting effect on the LO2 cells in vitro experiments. PBDE-47, PBDE-99 and HBCD showed dose-response relation.

关 键 词：四溴联苯醚 五溴联苯醚 九溴联苯醚 六溴环十二烷 四溴双酚A LO_2细胞 

分 类 号：X171.5[环境科学与工程—环境科学]