急性胰腺炎大鼠血清TNF-α、VEGF的变化及意义  

Significance of the expression of TNF-α and VEGF in rats with acute pancreatitis

作　　者：牛威[1] 郭莲怡[1] 

机构地区：[1]辽宁医学院附属第一医院消化内科,辽宁锦州121001

出　　处：《中国现代医学杂志》2012年第27期5-7,共3页China Journal of Modern Medicine

摘　　要：目的探讨急性胰腺炎(AP)大鼠血清中肿瘤坏死因子-α(TNF-α)、血管内皮细胞生长因子(VEGF)的变化及意义。方法 60只SD雄性大鼠随机分成2组:急性胰腺炎组(AP组)和假手术组(SO组),每组分为3个时间点(6 h、12 h、24 h),每个时间点10只大鼠;AP组经胰胆管注入3.5%牛磺胆酸钠(1 mL/kg)制作动物模型,SO组仅开腹后翻动胰腺。各组于相应时间点抽血并留取胰腺组织,检测血清TNF-α、VEGF、淀粉酶(AMY)含量,并观察各组胰腺组织病理变化。结果 AP组血清TNF-α、VEGF、AMY以及胰腺组织病理学评分较相同时间点SO组均显著升高(P<0.05);AP组各时间点血清VEGF与血清TNF-α及病理学评分均成正相关(P<0.05)。结论 TNF-α、VEGF在AP早期即开始升高,且与胰腺组织病理评分正相关,在AP过程中起重要作用,可作为AP早期的评测指标。[ Objective ] To observe the expressions of serum tumor necrosis factor-c~ （TNF-c~） and vascular en- dothelial growth factor （VEGF） in the rats with acute pancreatitis. [Methods] 60 Sprague-Dawley male rats were randomly divided into 2 groups： acute pancreatitis（AP） group and sham operation（SO） group, each group were divid- ed into three time points（6 h, 12 h, 24 h）, there are 10 rats at each time point. Rats of AP group were injected 3.5% sodium taurochalate （1 mL/kg） through the pancreatic duct. Rats of SO group were only ruffled the pancreas. To de- tect serum TNF-ot, VEGF, amylase （AMY） at corresponding time points and observe the pathological changes of the pancreatic tissue. [Results] Serum TNF-ot, VEGF, AMY, and pancreatic pathology score of the AP group were sig- nificantly higher than the SO group at the same time point （P 〈0.05）. Serum VEGF and TNF-c~ of AP group were correlated with pathology scores positively at each time point（P 〈0.05）. [ Conclusion] TNF-c~ and VEGF increase in the early phase of AP, correlated to pancreatic pathology score positively. They can be used as indicators in the e- valuation of early AP.

关 键 词：急性胰腺炎 肿瘤坏死因子-Α 血管内皮细胞生长因子 

分 类 号：R576.0[医药卫生—消化系统]