不同质量浓度苦草对铜绿微囊藻生长及抗氧化酶系统的影响  

Effects of different concentrations ofVallisneria spiralis Linn.on growth and antioxidant system of Microcystis aeruginosa

作　　者：陈国元[1] 李青松[1] 黄晓鸣[1] 朱木兰[1] 

机构地区：[1]厦门理工学院水资源环境研究所,厦门361024

出　　处：《环境工程学报》2012年第11期4107-4112,共6页Chinese Journal of Environmental Engineering

基　　金：厦门理工学院引进高层次人才科研启动项目(YKJ09022R);厦门市科技计划项目(3502Z 20110016);福建省教育厅A类资助项目(JA10252)

摘　　要：采用苦草(Vallisneria spiralis Linn.)和铜绿微囊藻(Microcystis aeruginosa)共生培养的实验方法,通过追踪测定铜绿微囊藻的生物量、叶绿素a含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性,研究了不同质量浓度苦草对铜绿微囊藻生长及抗氧化酶系统的影响。结果表明,质量浓度大于10 g/L时,苦草对铜绿微囊藻有明显的抑制作用,表现为苦草质量浓度为10、20和40 g/L时,第15天对铜绿微囊藻的抑制率分别为63.3%、94.7%和99.8%,培养过程中,铜绿微囊藻的叶绿素a含量逐渐减少,而SOD、POD活性及MDA含量呈现先增加后逐渐降低的趋势,表明苦草释放的化感物质在经过一定时间积累后能够明显抑制铜绿微囊藻SOD和POD的活性,引起细胞的氧化损伤,促进叶绿素的分解,从而导致藻类死亡,这是苦草抑制铜绿微囊藻生长的原因之一。The evaluation of different concentrations of Vallisneria spiralis Linn.on Microcystis aeruginosa＇s growth and antioxidant system was carried out through co-cultivation of Vallisneria spiralis Linn.and Microcystis aeruginosa by mornitoring the alga density,chlorophyll-a content,MDA content,SOD and POD activities.Results showed there was significant inhibition on Microcystis aeruginosa＇s growth when the co-cultivated Vallisneria spiralis Linn.＇s concentration was over 10 g/L.After 15 days incubation,the inhibition rates of Microcystis aeruginosa＇s growth were 63.3%,94.7% and 99.8% versus the concentration of Vallisneria spiralis Linn.at 10,20 and 40 g/L,respectively.During the incubation period,chlorophyll-a content of Microcystis aeruginosa decreased gradually,whereas MDA content,SOD and POD activities increased initially and decreased gradually thereafter.The study indicated that one of the mechanisms which led the inhibition of Microcystis aeruginos＇s growth by co-cultivation with Vallisneria spiralis Linn.demonstrated that the accumulated allelopathical substance which released from Vallisneria spiralis Linn.effectively inhibited SOD and POD activities of Microcystis aeruginos,induced the oxidative damage to cells and eventually facilitated chlorophyll degradation of Microcystis aeruginos.

关 键 词：苦草 铜绿微囊藻 共生培养 化感物质 

分 类 号：X171[环境科学与工程—环境科学]