吸烟对大鼠脑组织TNF-α表达的影响  

Effects of smoking on TNF-α expression in rats' brain

作　　者：庄严[1] 王丹丹[1] 柳忠兰[2] 曹亦宾[1] 陈丽丽[1] 

机构地区：[1]河北医科大学附属唐山工人医院神经内科,河北唐山063000 [2]中国医科大学附属第一医院神经内科

出　　处：《现代预防医学》2012年第21期5655-5656,共2页Modern Preventive Medicine

摘　　要：目的探讨吸烟对大鼠脑组织中肿瘤坏死因子-α(TNF-α)的影响。方法将48只Wister大白鼠随机分成3组,不吸烟组(A组),被动吸烟1个月组(B组)以及被动吸烟2个月组(C组)。通过免疫组织化学方法对比观察各组大鼠脑组织TNF-α的表达情况。结果不吸烟组大鼠TNF-α阳性率为6.25%,被动吸烟1月组大鼠阳性率为56.25%,被动吸烟2月组阳性率为81.25%,随着吸烟时间延长,TNF-α阳性率逐渐升高,各组比较差异有统计学意义(P﹤0.05);被动吸烟2个月组的大鼠TNF-α阳性细胞数为(37.57±3.42),明显高于被动吸烟1个月组和不吸烟组,且各组两两比较差异有统计学意义(P﹤0.05)。结论吸烟可引起脑组织中TNF-α表达增高,吸烟时间越长,TNF-α表达越多,进而加重血管炎性反应,增加脑血管疾病的发生。OBJECTIVE To investigate the effect of smoking on tumor necrosis factor-alpha（TNF-alpha） rats＇ brain tissue.METHODS 48 Wister rats were randomly divided into three groups,non-smoking group（group A）,passive smoking for one month（group B）,passive smoking for two months（group C）.Comparatively observed TNF-α expression in rat brain tissue by immunohistochemical methods.RESULTS The positive rate of TNF-α was 6.25% in non-smoking group,which in group B was 56.25% and 81.25% in group C.With the smoking time extended,TNF-α positive ratio increased,the difference in these groups was statistically significant（P ﹤ 0.05） ;positive cell count of rat TNF-α was 37.57 ± 3.42 in group C,significantly higher than that of group A and group B,and the difference was statistically significant in each group（P﹤0.05）.CONCLUSION Smoking can cause increased expression of TNF-α in brain tissue.The longer smoking time has,the more expression of TNF-alpha shows,thereby aggravating the vascular inflammatory response and increasing the occurrence of cerebrovascular disease.

关 键 词：被动吸烟 肿瘤坏死因子 大鼠 脑组织 

分 类 号：R743[医药卫生—神经病学与精神病学]