ATP生物发光测定试剂研究进展  

Reserach progress on ATP bioluminescence reagent

作　　者：吴慧清[1] 李程思[1] 吴清平[1] 张菊梅[1] 

机构地区：[1]广东省微生物研究所、广东省菌种保藏与应用重点实验室、广东省微生物应用新技术公共实验室、广东省华南应用微生物重点实验室省部共建国家实验室培育基地,广东广州510070

出　　处：《微生物学通报》2012年第11期1661-1667,共7页Microbiology China

基　　金：广东省科技计划项目(No.2010B030900013,2011A090200100)

摘　　要：萤火虫荧光素酶是ATP生物发光试剂的关键组成部分,可通过萤火虫尾提取纯化或基因工程技术制备,酶的活力和纯度决定了ATP生物发光试剂的性能。迄今许多先进技术在ATP生物发光试剂的制备中均有应用,包括酶基因工程改造技术、ATP循环的酶法放大技术、荧光素酶蛋白的活力及发光稳定技术,特异的细胞ATP提取技术等。ATP生物发光试剂的研究焦点主要集中在提高发光试剂的检测灵敏度和性能、增加产品的适应性等方面。Firefly luciferase is the key component of ATP bioluminescence reagent, gained from firefly lantern throuh extraction and purification or preparation through genetic engineering, the performance of ATP bioluminescence reagent was decided by the vitality and the purity of firefly luciferase. Up to now, many present advanced technology were applied on preparation the reagent such as genetic engineering, ATP amplification device, stabilization technology of luciferase protein and luminescence, and so on. Now research focus on improving detection sensitivity and luminescence performance of the ATP bioluminescence reagents, further raising the adaptability ofATP bioluminescence reagents.

关 键 词：ATP生物发光试剂 萤火虫荧光素酶 制备研究 

分 类 号：Q93-3[生物学—微生物学]