蛇床子素诱导急性脊髓损伤家兔阴茎勃起的实验研究  

Efficacy of osthole for inducing penile erection in rabbits with acute spinal cord injury

作　　者：周福荣[1] 孙建鹰[1] 周稳[1] 文莉东[1] 

机构地区：[1]成都市第六人民医院泌尿外科,四川成都610051

出　　处：《西部医学》2012年第11期2064-2066,共3页Medical Journal of West China

摘　　要：目的探讨蛇床子素诱导家兔阴茎勃起的作用及其机制。方法检测蛇床子素对磷酸二酯酶5(PDE5)的抑制活性;检测蛇床子素对离体阴茎海绵体组织产生cGMP的诱导活性;建立急性脊髓损伤家兔模型,蛇床子素静脉注射给药(3mg/kg或10mg/kg),通过测量暴露的阴茎粘膜长度评价蛇床子素体内诱导勃起的功能。结果蛇床子素对PDE5直接抑制活性IC50为88.9nmol/L;对离体阴茎海绵体cGMP诱导活性ED50为58.2μmol/L;在一氧化氮供体硝普钠存在条件下,静脉注射蛇床子素对ASCI家兔模型具有剂量依赖性促进阴茎勃起作用。结论蛇床子素对勃起功能障碍具有治疗价值。Objective The aim of this study was to investigate the effects of Osthole （OST） on inducing penile e- rection in rabbits with an acute spinal cord injury （ASCI）. Methods OST was given intravenously （3 or 10mg/kg） to conscious male albino rabbits with a surgical transection of the spinal cord at the L2-L4 lumbar vertebra. Erection was e- valuated in a time-course manner by measuring the length of the uncovered penile mucosa. Results The efficacy of OST was potentiated by an intravenous injection of sodium nitroprusside, a nitric oxide donor. Potentiation of the effeet by ni- tric oxide donor implies that OST can enhance the erectile activity during sexual arousal. Conclusion These results suggest that OST may be useful for treating erectile dysfunction.

关 键 词：磷酸二酯酶 蛇床子素 阴茎勃起 家兔 脊髓损伤 

分 类 号：R-33[医药卫生]