发酵豆粕替代鱼粉对黑鲷胃肠道和血清指标的影响  

Effects of fermented soybean meal replacing fish meal on gastrointestinal tract and serum indexes in black sea bream

作　　者：彭翔[1,2] 宋文新[1] 周凡[1,3] 肖金星[1,4] 章亭洲 邵庆均[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310058 [2]中国水产科学研究院,北京100141 [3]浙江省水产技术推广总站,浙江杭州310012 [4]浙江大学舟山海洋研究中心,浙江舟山316021 [5]浙江科峰生物技术有限公司,浙江嘉兴314423

出　　处：《江苏农业学报》2012年第5期1096-1103,共8页Jiangsu Journal of Agricultural Sciences

基　　金：国家十二五科技支撑计划项目(2011BAD13B08);国家海洋局海洋公益性行业专项(201005013);浙江省科技厅重大农业科技专项(2011C12054)

摘　　要：采用室内流水养殖系统饲养黑鲷(Acanthopagrus schlegelii)幼鱼56 d,研究了黑鲷饲粮中发酵豆粕替代鱼粉的效果。选取初始体重为(11.82±0.32)g的健康黑鲷,随机分为6组,每组3个重复,每个重复25尾。用发酵豆粕分别替代0(S0)、10%(S10)、20%(S20)、30%(S30)、40%(S40)和50%(S50)的鱼粉蛋白质。结果表明,S0、S10和S20组中黑鲷的胃肠组织结构没有明显损伤,替代水平高于20%则出现不同程度的胃肠结构损伤,S40组中肠结构严重损伤,S50组前肠和中肠结构严重损伤。S10、S20组蛋白酶和脂肪酶活性显著提高,各组淀粉酶活性变化不大。随着发酵豆粕替代水平的增加,血清葡萄糖(GLU)含量、过氧化物歧化酶(SOD)活性、溶菌酶(LZ)含量逐渐降低,而胆固醇(T-CHO)、谷草转氨酶(GOT)和谷丙转氨酶(GPT)含量逐渐升高。结果提示,饲粮中发酵豆粕替代20%鱼粉蛋白质可提高蛋白酶和脂肪酶活性,增强黑鲷抗氧化和免疫力,对消化道无不良影响。An 8-week feeding trial was conducted to investigate the effects of fish meal（FM） substitution with fermented soybean meal（FSBM） in the diets of black sea bream（Acanthopagrus schlegelii）,in 18 indoor flow-through fiberglass tanks（25 fish per tank）.The healthy black sea bream [（11.82±0.32） g,mean initial body weight] was selected and divided randomly into six groups and each group in triplicate tanks with 25 fish.Six experimental diets were formulated according to FSBM protein replacing fish meal protein at 0（S0）,10%（S10）,20%（S20）,30%（S30）,40%（S40）,50%（S50） levels,respectively.Each diet was randomly assigned to triplicate groups of fish twice daily.The results showed that there was no significant damage to the structure of gastrointestinal tract in S0,S10 and S20 groups,but damage appeared with the substitution level more than 20%.Midgut had a serious damage in S40 group as well as foregut and midgut in S50 group.There were significant increases in activities of protease and lipase in S10 and S20 groups,however,no significant variation on amylase.As the replacement level increased,the contents of serum glucose（GLU）,the activity of superoxide dismutase（SOD）,and the content of lysozyme（LZ） were decreased,while the contents of total cholesterol（T-CHO）,glutamic-oxalacetic transaminease（GOT） and glutamic-pyruvic transaminase（GPT） rose gradually.The results indicated that FSBM replacing FM protein at 20% level in diets could improve the activities of protease and lipase,enhance the antioxidant capacity and immunity of black sea bream,without impact on gastrointestinal tract.

关 键 词：黑鲷 发酵豆粕 胃肠道 血清指标 

分 类 号：S965.231[农业科学—水产养殖]