可储存介孔氧化锆-磷酸钙根管充填糊剂的实验研究  

Experimental research of storable mesoporous zirconia-doped calcium phosphate cement for root canal sealer

作　　者：许超[1] 迟小娟[1] 孙婧婧[1] 宋玉兴[1] 冯祖德[1] 

机构地区：[1]厦门大学材料学院,福建厦门361005

出　　处：《生物医学工程与临床》2012年第6期527-529,F0003,共4页Biomedical Engineering and Clinical Medicine

基　　金：福建省科技重大专项(2005HZ01-3);福建省发改委项目

摘　　要：目的制备可储存介孔氧化锆-磷酸钙根管充填糊剂(简称糊剂),并对其显影效果、理化性能、可储存性能及生物相容性进行评价。方法制备具有有序六方孔结构的钛稳定介孔氧化锆,并将其(质量分数5%、10%、15%、20%)与自固化磷酸钙骨水泥粉末混合制得介孔氧化锆-磷酸钙根管充填糊剂。通过X射线拍摄评价糊剂的显影效果;通过测试收缩率、薄膜厚度、流动性和溶解性评价糊剂的理化性能;通过测试不同储存时间糊剂的流动性能评价其可储存性;通过细胞毒性实验、口腔致敏动物实验、皮下组织埋植动物实验对糊剂进行生物安全性评价。结果①含有质量分数15%介孔氧化锆-磷酸钙根管充填糊剂显影效果满足临床需要;②所制糊剂的各项理化性能均符合ISO 6876中关于根管充填糊剂理化性能的评价标准;③所制糊剂可在4℃、50%相对湿度环境下长期密封保存;④所制糊剂材料对L-929细胞无毒性,无口腔黏膜刺激反应,不会对皮下组织产生毒害作用。结论可储存介孔氧化锆-磷酸钙根管充填糊剂具有良好的显影性能、理化性能、可储存性能及生物安全性,对根管充填的临床应用具有积极意义。Objective To prepare a storable mesoporous zirconia-doped calcium phosphate cement （CPC） for root canal sealer, and evaluate its performances of radiopacity, physicochemical properties, storability and biocompatibility. Methods Titanium- stabilized mesoporous zirconia（5 %, 10 %, 15 %, 20 %） with hexagonally ordered pore array was prepared and mixed with bone CPC powders, thus the mesoporous zirconia-doped CPC for root canal sealer was completely prepared. The radiopacity of the paste were evaluated using X-ray film. The physicochemical properties of the paste were evaluated by contractibility, film thickness, fluidity and dissolubility. The storability of the paste was evaluated by flow property of samples with different storage time. The biocompatibilities were evaluated by MTT, animal oral sensitizations and animal subcutaneous tissue implantations experiments. Results ①The paste included 15 % mesoporous zirconia showed improved development effects. ②The paste could be stored in the condition of 4 ℃, 50 % relative humidity for long term, the phase composition and particle morphology showed stability. ③The various physical and chemical properties of the paste were in accordance with the evaluation criterion of ISO 6876. ④The paste had no eytotoxicity to the L-929 cells as well as oral mucosa and subcutaneous tissue. Conclusion The storable mesoporous zirconia- doped CPC for root canal sealer showed well radiopacity, physicochemieal properties, storability and biosafety which are promising for the clinical applications of root canal filling.

关 键 词：介孔氧化锆 磷酸钙 根管充填糊剂 显影性能 可储存性能 生物相容性 

分 类 号：R781.05[医药卫生—口腔医学]