微波对体外培养视网膜神经细胞损伤的光镜与电镜观察  

Light and Electron Microscopic Observation on the Damage of Retinal Nervous Cells Cultured in vitro Induced by Microwave

作　　者：刘学东[1] 龚书明[1] 杨瑞华[1] 陈景元[1] 刘秀红[1] 邓中荣[1] 

机构地区：[1]第四军医大学军队卫生学教研室,西安市710032

出　　处：《工业卫生与职业病》2000年第3期129-131,共3页Industrial Health and Occupational Diseases

摘　　要：为了探讨 2 45 0MHz微波对眼底的损伤 ,采用体外培养视网膜神经细胞 ,按微波强度分为 3组进行微波辐照 1h ,进行光镜和透射电镜观察。结果表明 ,30mW /cm2 微波辐照可使视网膜神经细胞发生凋亡 ,6 0mW /cm2 微波辐照可导致细胞坏死。可见 ,微波可损伤体外培养的视网膜神经细胞 ,诱发细胞凋亡 ,随着微波辐照强度的增大 ,损伤程度加重。To study the damage of ocular fundus induced by microwave,retinal nervous cells were cultured in vitro and divided into 3 groups according to microwave intensity.The cell morphology was observed by light and electron microscopes after exposed to microwave for 1 hour.The results showed that the retinal nervous cells underwent apoptosis after exposed to 30 mW/cm 2 microwave,some portion of cells showed necrotic after exposed to 60 mW/cm 2 microwave.It suggested that the retinal nervous cells could be damaged and underwent apoptosis due to microwave exposure,and the damage was consistent with the microwave intensity.

关 键 词：微波 光镜 透射电镜 视网膜神经细胞损伤 

分 类 号：R774.6[医药卫生—眼科]