腺病毒介导的CD基因在人胰腺癌细胞中的靶向性表达  

Targeting Expression of Cytosine Deaminase Gene by Adenovirus mediated Transfer in Human Pancreatic Carcinoma Cells

作　　者：张世能[1] 袁世珍[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院消化内科,广东广州510120

出　　处：《中山医科大学学报》2000年第3期176-178,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：中山医科大学科研基金资助课题!(97112)

摘　　要：【目的】探讨腺病毒介导的大肠杆菌胞嘧啶脱氨酶 (E .coliCD)基因在胰腺癌细胞中靶向性表达的可能性及其作用。【方法】将含癌胚抗原 (carcinoembryonicantigenCEA)启动子的重组腺病毒感染人胰腺癌SW1990细胞 (CEA+ )、Capan 2细胞 (CEA-)和人宫颈癌Hela细胞 ,观察CD基因在 3种细胞中的表达及细胞对 5 FC的敏感性差异。【结果】腺病毒介导CD基因仅在SW1990细胞中呈专一性表达 ,转导CD基因的SW1990细胞对 5 FC的敏感性明显提高。【结论】含CEA启动子的CD基因疗法可能是胰腺癌靶向性基因治疗的可行途径。Objective To explore the possibility of the targeting expression of Escherichia coli cytosine deaminase(CD) gene by adenovirus-mediated transfer in human pancreatic carcinoma cells. Methods] Recombinant adenoviruses containing a carcinoembryonic antigen(CEA) promoter were transiently introduced into SW1990、 Capan-2 and Hela cells respectively. These cells were analyzed for gene expression and sensitivity to 5 fluorocytosine(5 FC). Results A specific expression of cytosine deaminase gene by adenovirus-mediated transfer exibited only in SW1990 cells. Transduction of the vector containing CEA promoter cytosine deaminase gene resulted in significant increase of sensitivity of SW1990 cells (CEA producing) to 5 FC. Conclusion These results demonstrate that utilization of the CEA promoter in an adenoviral vector may be a potential approach for targeting gene therapy of pancreatic carcinoma.

关 键 词：胰腺肿瘤 腺病毒 CD基因 基因治疗 靶向性表达 

分 类 号：R735.905[医药卫生—肿瘤] R730.59[医药卫生—临床医学]