膜联蛋白A2在炎症性肠病肠粘膜中的表达及临床意义  

Annexin A2 expression in intestinal mucosa of patients with inflammatory bowel disease and its clinical implications

作　　者：张朝霞[1] 赵芯梅[1] 吕超蓝[1] 李晨[1] 智发朝[1] 

机构地区：[1]南方医科大学南方医院消化内科//省胃肠疾病重点实验室,广东广州510515

出　　处：《南方医科大学学报》2012年第11期1548-1552,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81170341)~~

摘　　要：目的探讨膜联蛋白A2(ANXA2)在炎症性肠病(IBD)肠粘膜组织中的表达及临床意义。方法免疫组织化学、荧光定量PCR方法检测ANXA2在54例溃疡性结肠炎(UC)、37例克罗恩病(CD)及15例正常肠粘膜组织中的表达,并分析其表达与IBD临床、病理及内镜参数间的关系。结果 ANXA2在UC的阳性表达显著高于CD及正常对照组(P<0.05),且UC中临床表现严重、内镜分级高的患者较临床表现轻、内镜分级低者表达显著升高(P<0.05)。ANXA2在CD的表达较正常对照组低(P<0.05),其表达与CD的临床病理参数无相关性(P>0.05)。与正常对照及CD相比较,ANXA2 mRNA表达量在UC中明显升高(P<0.05)。结论 ANXA2可作为IBD的诊断与鉴别诊断指标,ANXA2表达在UC发生发展中可能起着重要作用,有望作为其早期诊断及靶向治疗的分子标记。Objective To explore the role of annexin A2 （ANXA2） expression in the intestinal mucosa in the pathogenesis of inflammatory bowel disease （IBD）. Methods Intestinal or colonic mucosal biopsy samples were obtained from 54 patients with ulcerative colitis （UC）, 37 with Crohn＇s disease （CD）, and 15 healthy control subjects. Immunohistochemistry was employed to examine the expression of ANXA2 in the intestinal mucosa, and mRNA expression of ANXA2 was detected using real-time PCR. Results Immunohistochemistry showed a ANXA2 positivity rate of 83.3% （45/54） in patients with UC, 27.0% （10/37） in patients with CD, and 53.3% （8/15） in the control subjects. ANXA2 expression in the intestinal or colonic mucosa was significantly up-regulated in patients with UC compared with the patients with CD and healthy control subjects, but was significantly lower in patients with CD than in the healthy controls （P〈0.05）. The expression levels of ANXA2 were strongly associated with the severity of clinical manifestations and the histopathological grades of UC （P〈0.05）. Compared with the healthy controls and patients with CD, patients with UC showed a significantly increased ANXA2 mRNA expression level in the inflamed mucosa of UC （P〈0.05）. Conclusion ANXA2 can serve as a marker for differential diagnosis of IBD, and its up-regulated expression is closely related to the pathogenesis of UC.

关 键 词：膜联蛋白A2 炎症性肠病 溃疡性结肠炎 克罗恩病 

分 类 号：R574.62[医药卫生—消化系统]