基于表达谱分析识别鼻咽癌血管生成及淋巴管生成的调控通路  

Analysis of angiogenesis and lymphangiogenesis signaling pathways based on gene expression patterns of nasopharyngeal carcinoma

作　　者：马佳怡[1] 黄坤[1] 黄仲曦[2] 

机构地区：[1]南方医科大学第一临床医学院2006级八年制,广东广州510515 [2]南方医科大学肿瘤研究所,广东广州510515

出　　处：《南方医科大学学报》2012年第11期1553-1558,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(90612020)~~

摘　　要：目的探讨鼻咽癌是通过调节哪些靶基因的表达影响血管生成和淋巴管生成。方法以Sengupta等通过基因芯片数据(其中含10例正常鼻咽组织,31例鼻咽癌组织)分析所得的831个鼻咽癌与正常鼻咽组织差异表达基因为基础,根据最新的基因组信息以及设定差异表达值的最小阈值260,筛选出246个有效基因。对这246个基因进行基于文献挖掘的基因功能分析和网络构建,最后进行通路分析。结果 246个基因与鼻咽癌、EB病毒、转移、血管生成、淋巴管生成、侵袭等关键词特异相关。其中,52个基因已知与血管生成相关(P=0.00001),19个基因构成了血管生成基因网络(P=0.0042);21个基因已知与淋巴管生成相关(P=0.00001),6个基因构成了与淋巴管生成相关基因网络(P=0.0226)。包含PTGS2在内的8个基因参与NFκB信号通路已知在小细胞肺癌中与血管生成密切相关(P=7.87E-07)。包含STAT1及CXCL10等在内的5个基因参与了Toll样受体通路(P=0.00176)。结论 PTGS2和NFκB共同调控鼻咽癌血管生成,Toll样受体信号通路可能与淋巴管生成密切相关。它们的相互作用方式值得进一步研究。Objective To pinpoint angiogenesis. and lymphangiogenesis-related genes in nasopharyngeal carcinoma （NFC）. Methods Based on the reported microarray data which identified 831 differentially expressed genes in NPC tissues and the latest genomic information, we selected 246 genes for analysis with the smallest differential expression threshold of 260. Gene function analysis and network construction was carried out based on literature mining for analysis of the signaling pathways related with angiogenesis and lymphangiogenesis of NPC. Results The 246 genes were related with such keywords as nasopharyngeal carcinoma, EB virus, metastasis, angiogenesis, lymphangiogenesis, and invasion. Particularly, we found that up to 52 genes were associated with angiogenesis （P=0.00001）, and 19 genes form 12 related gene pairs （P=0.0042）. Twenty-one lymphangiogenesis-related genes were identified （P=0.00001）, and 6 of these genes formed a gene network （P=0.0226）. Eight genes, including PTGS2, participated in the nuclear factor-KB （NF-kB） pathway, which was closely related to angiogenesis in small cell lung cancer （P=7.87E-07）. Five genes, including STAT1 and CXCL10, participated in toll-like receptor signaling pathway （P=0.00176）. Conclusion PTGS2 and NF-kB promote angiogenesis of NPC, and the role of toil-like receptor signaling pathway in lymphangiogenesis warrants further investigation.

关 键 词：鼻咽癌 基因芯片 数据挖掘 信号通路 血管生成 淋巴管生成 

分 类 号：R739.63[医药卫生—肿瘤]