膀胱癌患者尿中肝素含量检测及其临床意义  

Determination of Urinary Heparin in Bladder Transitional Cell Carcinoma and Its Clinical Significance

作　　者：李杰[1] 眭元庚[1] 徐正铨[1] 吴宏飞[1] 程双管[1] 张炜[1] 丁益[2] 

机构地区：[1]南京医科大学第一附属医院泌尿外科研究室,南京210029 [2]南京大学医药生物技术国家重点实验室,南京210008

出　　处：《南京医科大学学报（自然科学版）》2000年第3期170-172,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的　建立一种高效毛细管电泳检测尿液中排泄肝素的方法 ,并用于检测膀胱肿瘤患者尿中的肝素排泄量。方法　采用磷酸缓冲体系的反极性毛细管电泳法 ,电压为 - 2 0 k V,检测波长为 190 nm,并用其测定 2 1例膀胱肿瘤患者及 12例健康者尿中排泄的肝素含量。实验数据经方差分析 ,显著性水平α=0 .0 5。结果　肝素峰面积和其含量呈良好的线性关系 ,尿中肝素排泄量 12例健康者为 (0 .94± 0 .48) μg/ L,2 1例膀胱肿瘤者中 11例 Ta～T1 期患者为 (1.30± 0 .73) μg/ L,10例 T2 ～T4期患者为 (0 .5 4± 0 .45 )μg/ L ,T2 ～ T4期者比 Ta～ T1 期及健康者显著降低 (P<0 .0 5 )。结论　反极性毛细管电泳法是一种分离人尿中肝素高效、快速、简便的方法 ,对膀胱肿瘤患者尿中肝素的检测发现 ,其排泄量的降低与膀胱肿瘤的浸润转移有关。Objective To establish a capillary electrophoresis (CE) method to determine the value of urinary heparin in bladder transitional cell carcinoma (BTCC).Methods Reverse polarity CE with a UV detector 190 nm in a -20kV and pH2.5 buffer system was used to determine urinary heparin in 21 patients with BTCC and 12 normal individuals. The analysis of variance (ANOVA) was used in this experiment and significance level is 0.05.Results The correlation with heparin peak area and its value is linear. The value of urinary haparin is (0.94±0.48) μg/L in 12 normal, (1.30±0.73) μg/L in 11 patients with Ta～T 1 BTCC and (0.54±0.45) μg/L in 10 patients with T 2~T 4 BTCC. Urinary heparin was lower in T 2~T 4 BTCC than in normal and Ta^T 1 BTCC ( P <0.05).Conclusion Reverse polarity CE is a high resolution, rapid and simple method to seperate heparin from urine. Falling of urinary heparin in BTCC plays an important role in the infiltration and transition of the carcinoma cells.

关 键 词：膀胱肿瘤 肝素 毛细管电泳 尿液 

分 类 号：R737.14[医药卫生—肿瘤]