一种高灵敏喷射式Ru(bpy)_3^(2+)电化学发光流动体系的构建  

Development of Sensitive Jet Ru(bpy) 32+ Electrochemiluminescence Flow System

作　　者：陈钰[1] 王捷[1] 刘仲明[1] 

机构地区：[1]广州军区广州总医院医学实验科,广州510010

出　　处：《医疗卫生装备》2012年第10期12-14,共3页Chinese Medical Equipment Journal

基　　金：广东省教育部学产研结合项目(2010B090400187);传感技术联合国家重点实验室基金课题(Skt003)

摘　　要：目的:在改进的喷射式电化学发光流动注射系统的基础上,构建了一种高灵敏的Ru(bpy)32+电化学发光体系。方法:考察非离子表面活性剂Triton X-100、Tween-20以及共反应物TPA、DBAE对Ru(bpy)32+电化学发光体系的影响。结果:建立了含有0.05%Triton X-100或0.1%Tween-20添加剂的Ru(bpy)32+-DBAE喷射式电化学发光体系,该体系检测范围宽、检测灵敏度高达5×10-15mol/L。结论:该体系比常规Ru(bpy)32+-TPA体系具有更高的稳定性和环境友好性,有望用于后续临床免疫、基因电化学发光检测系统的建立。Objective To develop a sensitive Ru（bpy） 32＋ electrochemiluminescence（ECL） system based on the improved jet ECL flow injection system.Methods The effects of non-ionic surfactants Triton X-100,Tween-20 and co-reactant Tripropylamine（TPA）,2-（Dibutylamino） ethanol（DBAE） on the Ru（bpy） 32＋ ECL system were studied.Results It was found that Ru（bpy） 32＋-DBAE system had a wide detection range and the detection limit was as high as 5×10-15 mol / L,with adding 0.05% Triton X-100 or 0.1% Tween-20.Conclusion Compared with Ru（bpy） 32＋-TPA ECL system,the Ru（bpy）32＋-DBAE system has a higher stability and environmental friendliness,and is expected to be used for subsequent clinical immunology and gene ECL detection system.

关 键 词：喷射式 流动池 电化学发光 添加剂 增敏 DBAE 

分 类 号：O657.3[理学—分析化学]