子宫内膜癌中p16基因甲基化与表达的研究  

Methylation and expression of the p16 gene in endometrial carcinoma

作　　者：晁红霞[1] 孙建衡[1] 陆士新[1] 

机构地区：[1]中国医学科学院

出　　处：《中华肿瘤杂志》2000年第3期228-231,共4页Chinese Journal of Oncology

摘　　要：目的　研究p16基因甲基化状态及其p16基因表达异常与子宫内膜癌发生发展的关系。方法　采用限制性内切酶酶切、PCR及RT PCR检测子宫内膜组织p16基因 5′CpG岛甲基化状态及p16基因mRNA表达。结果　 8例正常子宫内膜无甲基化 ,且p16mRNA表达正常。 6例子宫内膜非典型增生中 ,有 1例甲基化 ;38例子宫内膜癌中 ,13例甲基化 ,占 34 .2 %。 6例子宫内膜单纯及复合增生中 ,有 5例p16mRNA高表达 ;6例子宫内膜非典型增生中 ,4例p16mRNA表达下降或无表达 ;38例子宫内膜癌中 ,2 7例p16mRNA表达下降或无表达 ,占 71 1%。结论　p16基因甲基化是子宫内膜癌发生的早期事件 ,与子宫内膜癌的发生发展有关。Objective To analyse the relationship between methylation status of the 5′ CpG island and mRNA expression of the p16 gene in endometrial carcinoma.Methods Methylation status of p16 gene was determined by methylation sensitive restriction enzymes, PCR and p16 mRNA expression was evaluated by RT PCR in a series of 8 specimens with normal endometrium(NE), 6 with simple and complex hyperplasia(SCH), 6 with atypical hyperplasia (AH) and 38 with endometrial carcinoma(EC).Results Eight specimens of NE displayed no methylation and showed normal expression of the p16 mRNA. In 1 of the 6 AH and 13 of the 38(34.21%) EC, there was methylation of p16 exon 1; 5 of the 6 SCH showed overexpresion of p16 mRNA, 4 of the 6 AH and 27 of the 38 (71.05%) EC exhibited decrease or loss of p16 mRNA expression.Conclusion Hypermethylation of p16 gene is an early event of endometrial carcinogenesis and is associated with the progression of endometrial carcinoma. Hypermethylation is hightly correlated with inhibition of p16 gene transcription.

关 键 词：子宫内膜肿瘤 P16基因 甲基化 基因表达 

分 类 号：R737.33[医药卫生—肿瘤]