伯氏嗜线虫致病杆菌蛋白酶抑制剂Xbpi-1对豌豆蚜Acyrthosiphon pisum Harris生物活性的研究  

Biological Activity of a Protease Inhibitor from Xenorhabdus bovienii Strain BJFS526 against Pea Aphid Acyrthosiphon pisum Harris

作　　者：金丹娟[1,2] 薛仁风[3] 张河庆[2] 曾凡荣[2] 董双林[1] 

机构地区：[1]南京农业大学植物保护学院昆虫系/教育部农作物病虫害综合治理重点实验室,南京210095 [2]中国农业科学院植物保护研究所/农业部作物有害生物综合治理重点实验室,北京100081 [3]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《中国生物防治学报》2012年第4期508-513,共6页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30871673)

摘　　要：蛋白酶抑制剂是一类能够抑制蛋白水解酶活性的小分子蛋白质,在昆虫肠道内通过抑制蛋白酶的活性,可以干扰昆虫的正常生长和发育。本文利用体外重组表达的蛋白酶抑制剂Xbpi-1,研究了Xbpi-1对豌豆蚜Acyrthosiphon pisum Harris的杀虫活性及其生化机制。结果表明,取食含蛋白酶抑制剂Xbpi-1浓度为50、200、400、800μg.mL 1人工饲料的豌豆蚜,96 h后的校正死亡率分别为2.1%、9.4%、19.7%和50.9%,虫重分别减少12.3%、20.3%、25.7%和33.7%。生化测定结果表明,4种浓度处理的豌豆蚜体内总蛋白酶、氨肽酶、胰蛋白酶、胰凝乳蛋白酶的活性均有显著降低,浓度为800μg.mL 1时,其酶活性分别降低了29.6%、26.1%、23.5%和23.8%。本研究结果说明,蛋白酶抑制剂Xbpi-1对豌豆蚜具有较好的生物活性,为进一步开发应用于豌豆蚜等刺吸式害虫的防治提供了理论依据。Protease inhibitors are capable of affecting insect survival and development by inhibition of the activities of proteases. In this study, the biological activity of recombinant protease inhibitor Xbpi-1 against the pea aphid, Acyrthosiphon pisum Harris, and the effects on the activities of aphid proteases were evaluated. The pea aphids fed on artificial diet containing 50, 200, 400, 800 μg·mL^-1 Xbpi-1 protein showed calibrated mortality of 2.1%, 9.4%, 19.7%, 50.9%, and aphid weights were reduced 12.3%, 20.3%, 25.7%, 33.7%, respectively compared with those of the control. The results showed that the activities of pea aphid proteases （trypsin, chymotrypsin, leucine aminopeptidase and total protinase） were significantly inhibited by the protease inhibitor protein. The results indicated that the protease inhibitor protein used in this study may be a potential bio-pesticide for controlling of sucking insect pests.

关 键 词：伯氏嗜线虫致病杆菌 蛋白酶抑制剂 豌豆蚜 杀虫活性 蛋白酶活性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] S436.3[农业科学—植物保护]