哈茨木霉发酵液中peptaibols抗菌肽的鉴定及活性研究  

Identification of Antibiotic Peptaibols from Fermentation Broth of Trichoderma harzianum

作　　者：潘顺[1] 刘雷[2] 王为民[1] 

机构地区：[1]浙江省生物计量及检验检疫技术重点实验室/中国计量学院生命科学学院,杭州310018 [2]澳大利亚南十字星大学植物科学研究中心

出　　处：《中国生物防治学报》2012年第4期528-536,共9页Chinese Journal of Biological Control

基　　金：浙江省科技厅重大科技专项(2008C02007-2)

摘　　要：通过大孔吸附树脂、葡聚糖凝胶、薄层制备硅胶板(TLC)及制备高效液相色谱(HPLC)从哈茨木霉菌Trichoderma harzianum发酵液中分离、纯化出4种由20个氨基酸组成的线性peptaibols抗菌肽FD1、FD2、FD3、FD4。经ESI-IT-MS串联质谱鉴定,其分别为抗菌肽Alamethicin F-50、Atroviridin B、Alamethicin II和Atroviridin C。菌丝生长及孢子萌发实验表明其对水稻纹枯病菌Rhizoctonia solani AG4、黄瓜立枯病菌R.solani、黄瓜枯萎病菌Fusarium oxysporum f.sp.cucumerinum、西瓜枯萎病菌F.oxysporum f.sp.niveum、水稻稻瘟病菌Magnaporthe oryzae具有较好的抑制作用,同时发现木霉素Trichodermin与peptaibols抗菌肽Alamethicin F-50联合作用时对菌丝生长具有协同抑制作用。Four microheterogenous peptaibol antibiotics containing 20 amino acids were isolated from fermentation broth of Trichoderma harzianum and purified by polystyrene resin, sephadex, preparative thin-layer chromatography （TLC） and preparative high-performance liquid chromatography （HPLC）. They were identified as Alamethicin F-50, Atroviridin B, Alamethicin II and Atroviridin C with ESI-IT-MS2. Mycelial growth and spore germination experiments showed that the 4 compounds have strong inhibiting effects against Thanatephorus cucumeris, Rhizoctonia solani, Fusarium oxysporum f. sp. cucumerinum, F. oxysporum f. sp. niveum, Magnaporthe oryzae, and Alamethicin F-50 exerted synergistic antibiotic effect when applied with trichodermin on the tested plant pathogens.

关 键 词：哈茨木霉菌 抗菌肽 抑菌活性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]