正交试验优选丁香抑马拉色菌活性物质提取工艺  

Optimization of Extraction Technology for Anti-Malassezia Furfur Active Substances from Eugenia caryophyllataby Orthogonal Test

作　　者：宋杰[1] 董银卯[1] 孟宏[2] 

机构地区：[1]北京工商大学,北京100048 [2]中国中医科学院针灸研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第22期68-71,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选丁香中抑菌成分的提取工艺。方法:以糠秕马拉色菌为供试菌,通过管碟法测试抑菌效果,通过单因素试验和正交试验确定丁香提取物的最优提取工艺。结果:丁香最优提取工艺为70%乙醇回流提取,料液比1∶25,提取时间2 h,提取温度65℃。丁香醇提物对糠秕马拉色菌的抑菌圈直径为(23.08±0.46)mm,抑菌活力为高度敏感。结论:优选的提取工艺稳定可行。Objective： To optimize of extraction technology of antibacterial substances from Eugenia caryophyllata. Method： With malassezia furfur as test bacteria, antibacterial effect was tested by cup-plate method, extraction technology of extract from E. caryophyllata was optimized by single factor test and orthogonal test. Result： Optimum extraction technology was as following： refluxing extracted 2 h with 25 times the amount of 70% ethanol at 65℃. diameter of inhibition zone was （23. 08±0. 46） mm, antibacterial activity was hypersensitive. Conclusion： This optimized technology was stable and feasible.

关 键 词：丁香 糠秕马拉色菌 提取 抑菌 

分 类 号：R283.6[医药卫生—中药学]