响应面分析法优化蜂胶黄酮脂质体制备工艺  

Optimization of Preparation Conditions for Propolis Derived Flavonoid Liposomes by Response Surface Methodology

作　　者：袁菊[1] 张亚宁[1] 范云鹏[1] 胡元亮[1] 王远垒[1] 赵晓娟[1] 王德云[1] 

机构地区：[1]南京农业大学中兽医学研究室,江苏南京210095

出　　处：《食品科学》2012年第20期1-6,共6页Food Science

基　　金：国家自然科学基金青年科学基金项目(30901085);"十一五"国家科技支撑计划项目(2008BADB4B06)

摘　　要：目的:优选蜂胶黄酮脂质体制备的的最佳条件。方法:采用乙醇注入法制备蜂胶黄酮脂质体,在单因素试验基础上,以脂药比、膜材比、注入速度为影响因素,包封率为响应值,利用Box-Behnken试验设计原理,采用3因素3水平的响应面分析法,对蜂胶黄酮脂质体制备工艺进行优化。结果:蜂胶黄酮脂质体的最佳制备条件为脂药比9.6:1、膜材比8.5:1、注入速度0.8mL/min。结论:采用最佳条件制备的蜂胶黄酮脂质体包封率较高,验证实验测得蜂胶黄酮脂质体的包封率为91.67%,与模型预测值相对误差为0.086%,重现性好。Objective： To optimize conditions for the preparation of propolis derived flavonoid（PF） liposome.Methods： PF liposomes were prepared by ethanol injection method.One-factor-at-a-time design was used to investigate the effects of five process parameters on encapsulation efficiency.Three main process parameters that influence encapsulation efficiency including lecithin/PF mass ratio,lecithin/cholesterol mass ratio and injection speed were optimized by response surface analysis based on a three-variable,three-level Box-Behnken experimental design.Results： The optimal conditions for preparing PF liposomes were determined as 9.6：1 of lecithin/PF mass ratio,8.5：1 of lecithin/cholesterol mass ratio and 0.8 mL/min of injection speed.Conclusion： Under these conditions,the actual encapsulation efficiency of PF liposomes was 91.67%,which showed a relative error of 0.086% when compared with the predictive value.

关 键 词：响应面分析 蜂胶黄酮脂质体 包封率 乙醇注入法 脂药比 

分 类 号：S853.74[农业科学—临床兽医学]