铜盐-热变性法分离提取羊血超氧化物歧化酶工艺条件优化  

Optimization of Extraction Conditions for Superoxide Dismutase from Sheep Blood by Thermal Denaturation Coupled with CuSO_4

作　　者：杨学山[1] 付文力[1] 孔志亮[1] 杨孝朴[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《食品科学》2012年第20期42-45,共4页Food Science

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2010-03)

摘　　要：目的:优化铜盐-热变性法分离提取羊血超氧化物歧化酶(SOD)工艺。方法:采用单因素和正交试验,对影响SOD比活力的主要因素进行研究。结果:铜盐-热变性法分离提取SOD的最优工艺条件为热变性温度65℃、热变性时间15min、添加体积分数2.5%的10mmol/L CuSO4溶液,丙酮用量为溶血液的1.25倍,在此条件下得到的SOD比活力为2338.9U/mg。结论:此方法操作简单,成本低,适合工厂化生产。The purpose of this study was to optimize the extraction of superoxide dismutase（SOD） from sheep blood by thermal denaturation coupled with CuSO4.Main process conditions that influence SOD specific activity were optimized using one-factor-at-a-time and orthogonal array design.The results showed that thermal denaturation at 65 ℃ for 15 min in the presence of 2.5% CuSO4in 10 mmol/L aqueous solution and subsequent addition of a 1.25-fold excess of acetone resulted in an SOD specific activity of 2338.9 U/mg.This method was simple,low cost and suitable for industrialized production.

关 键 词：超氧化物歧化酶 酶活性 硫酸铜 热变性 

分 类 号：Q814[生物学—生物工程]