白血病细胞多巴胺受体基因甲基化研究  

Study on aberrant DNA methylation of dopamine D4 receptor gene in acute myeloid leukemia

作　　者：于力[1] 王全顺[1] 顾群[1] 史子江[1] 靳海杰[1] 

机构地区：[1]解放军总医院血液科,北京100853

出　　处：《军医进修学院学报》2000年第1期62-64,共3页Academic Journal of Pla Postgraduate Medical School

基　　金："九五"军队科研基金!项目 ( 98M14 1)

摘　　要：目的 :探讨多巴胺受体 ( DRD4 )基因第一外显子中 Not 酶切位点甲基化与急性非淋巴细胞白血病( AML)的关系。方法 :应用 Southern印记杂交及 DNA序列分析技术对 AML 患者骨髓标本进行分析。结果 :在 2 7例白血病患者骨髓标本中 16例 DRD4基因甲基化 ( 5 9% ) ,其中初治化疗敏感患者 DRD4基因甲基化发生率为5 0 % ( 9/18) ,复发及难治患者甲基化发生率为 78% ( 7/9) ,而 9例正常人骨髓标本 DRD4基因均无甲基化。结论 :DRD4基因甲基化在白血病患者发生率明显高于正常人 ( P<0 .0 5 ) ,而在复发及难治患者其发生率略高于初治化疗敏感患者 ,DRD4基因甲基化可能与白血病的发生。Objective:To investigate aberrant DNA methylation of Not I site on exon I of dopamine D4 receptor (DRD4) gene in acute myeloid leukemia (AML).Methods:Southern Blot and DNA sequencing technique were performed on DNA from AML bone marrow samples. Results:Sixteen samples from 27 AML patients (59%) showed DNA methylation of DRD4 gene. Nine samples from 18 diagnostic chemosensitive patients (50%) and 7 samples from 9 refractory and relapse AML patients (78%) showed DNA methylation. But 9 normal bone marrow samples did not show any DNA methylation on DRD4 gene. Conclusion:The incidence of DRD4 gene methylation in AML was significantly higher than that in normal bone marrow ( P <0 05). There is potential importance of DRD4 gene methylation in initiation and development of AML.

关 键 词：急性白血病 多巴胺受体 DNA甲基化 

分 类 号：R733.710.2[医药卫生—肿瘤]