外源NO对重金属Cd胁迫下亚麻幼苗叶片抗氧化能力的影响  

Effects of exogenous nitric oxide on antioxidant capability of seeding leaves in linseed under Cd stress

作　　者：徐严[1] 魏小红[1] 李兵兵[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2012年第5期45-49,55,共6页Journal of Gansu Agricultural University

基　　金：澳大利亚援助局资助项目(072-036007)

摘　　要：为探讨NO对CdCl2胁迫下的亚麻幼苗叶片抗氧化能力的影响,以‘天亚2号’为供试材料,采用不同浓度(0.1、0.5、1.0mmol/L)外源NO供体SNP处理0.1mmol/L CdCl2胁迫下的亚麻幼苗,分别于0(胁迫前)、24、48、72、96、120h后采集生长状况一致的亚麻幼苗叶片进行游离脯氨酸(Pro)含量、丙二醛(MDA)含量、过氧化氢(H2O2)含量、O2.-产生速率、过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性的测定.结果表明:外源NO可缓解Cd胁迫造成的亚麻幼苗膜质过氧化产物丙二醛含量的升高,促进脯氨酸积累,提高超氧化物歧化酶、过氧化氢酶和过氧化物酶的活性,并能抑制过氧化氢和超氧阴离子的产生.这种保护效应与NO的浓度明显相关,其中,0.1mmol/L SNP处理效果显著优于1.0mmol/L SNP处理.The influence of exogenous nitric oxide donor,SNP at concentrations of 0.1,0.5 and 1.0 mmol/L on antioxidant capability in linseed under 0.1 mmol/L CdCl2 stress was studied through 120 h of treatment.The results indicated that the activities of antioxidant enzymes were increased,such as superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT）,which were consistent with the up-regulation of the content of proline,and were consistent with the down-regulation of O·-2 production speed,the content of malondialdehyde（MDA） and H2O2 in linseed seedling leaves compared with CdCl2 stress.The protective ability of concentration of 0.1 mmol/L was significantly better than that of 1.0 mmol/L.

关 键 词：亚麻 外源NO CD胁迫 抗氧化能力 

分 类 号：S563.2[农业科学—作物学]