雌激素对卵巢癌细胞株体外生长的影响  

Influence of Estrogen on the Epithelial Ovarian Cancer Cell Lines in Vitro

作　　者：杨毅[1] 沈铿[1] 郎景和[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,100730

出　　处：《中华妇产科杂志》2000年第3期172-174,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨 17 β雌二醇 (E2 )对卵巢癌细胞株体外生长和细胞周期的影响。方法　采用四甲基偶氮唑蓝 (MTT)比色法和流式细胞术 (FCM)的方法 ,观察了卵巢癌细胞株CAOV3和OVCAR3在加入含有不同浓度 17 βE2 培养液后细胞活性和细胞周期的变化 ;同时采用免疫组化方法 ,测定癌细胞在加入 17 βE2 前后雌激素受体表达的变化。结果　 (1)浓度为 0 .2 5～ 5 .0 0nmol/L的 17 βE2 ,可轻度抑制卵巢癌细胞株的生长 ,抑制率为 6 .3%～ 36 .0 %。 (2 ) 17 βE2 可使CAOV3细胞株中G0 及G1期的细胞由 5 5 .0 %减少为 19.0 %～ 30 .0 % ,S期的细胞由 30 .0 %增加到 5 2 .0 % ,G2 及M期的细胞由14.0 %增加到 17.0 %～ 2 8.0 % ;使OVCAR3细胞中G2 及M期的细胞由 37.0 %减少为 2 2 .0 %～30 .0 % ,S期的细胞由 31.0 %增加到 38.0 %～ 5 1.0 %。 17 βE2 对G0 及G1期的OVCAR3细胞没有明显的影响 ,加入 17 βE2 前为 32 .0 % ,加入 17 βE2 后为 2 9.0 %～ 31.0 %。 (3)卵巢癌细胞株CAOV3和OVCAR3均为雌激素受体阳性细胞株 ,加入 17 βE2 对其雌激素受体的表达没有明显影响。结论　雌激素对卵巢癌细胞株CAOV3和OVCAR3没有刺激其生长的作用 ,相反可轻度抑制CAOV3和OVCAR3细胞的生长 。Objective To study the effect of 17 β estradiol on the growth of the epithelial ovarian carcinoma cell lines in vitro. Methods The proliferative capacity of the ovarian carcinoma cell lines CAOV3 and OVCAR3 in the cultrue medium with 17 β estradiol was evaluated by the means of the mircocultrue tetrazolium assay (MTT) and cell kinetics was analyzed by flow cytometry (FCM). The expression of estrogen receptor (ER) of the two cell lines was examined by the immunohistochemical staining. Results The growth of both two epithelial ovarian cancer cell lines was slightly inhibited by 17 β estradiol (0.25～5.00 nmol/L), and the inhibitive rate was 6.3%～36.0%. FCM showed that the kinetics of the two cell lines were obviously changed. Comparing with the kinetics of CAOV3 without estradiol, the cell rate of Stage G 0/G 1 of CAOV3 with estradiol decreased from 55.0% to 19.0%～30.0%, cell rate of Stage S increased from 30.0% to 52.0%, cell rate of Stage G 2/M increased from 14.0% to 17.0%～28.0%; In OVCAR3, cell rate of Stage G 2/M decreased from 37% to 22.0%～30.0%, cell rate of Stage S increased from 31.0% to 38.0%～51.0%, cell rate of Stage G 0/G 1 did not changed much (cell rate without estradiol was 32.0%, while cell rate with estradiol 29.0%～ 31.0%). Estrogen receptors of the two cell lines were positive and not changed after cultured in the medium with 17 β estradiol. Conclusion The proliferate capacity of the two epithelial ovarian cancer cell lines CAOV3 and OVCAR3 can be inhibited by the estrogen (0.25～5.00 nmol/L), possibly through the change of the tumor cell kinetics.

关 键 词：卵巢肿瘤 雌激素替代疗法 雌二醇 

分 类 号：R737.31[医药卫生—肿瘤]