Protein differential expression in the elongating cotton (Gossypium hirsutum L.) fiber under nitrogen stress  

作　　者：WANG YouHua ZHENG Mi GAO XiangBin ZHOU ZhiGuo 

机构地区：[1]College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Science China(Life Sciences)》2012年第11期984-992,共9页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30971735);the Specialized Research Funds for the Doctoral Program of Higher Education (Grant No.200803070017)

摘　　要：Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber development much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance.Nitrogen （N） is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess con-ditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spec- trometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber develop-ment much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance.

关 键 词：COTTON FIBER PROTEIN NITROGEN STRESS 

分 类 号：S563.1[农业科学—作物学] Q51[生物学—生物化学]