球形幽门螺杆菌致细胞空泡变性毒力研究  

STUDY ON THE VACUOLATING CYTOTOXICITY OF COCCOID HELICOBACTER PYLORI

作　　者：佘菲菲[1] 朱苹[1] 史碧山[2] 苏东辉[1] 强华[1] 陈月秀[1] 郑秀芬[1] 

机构地区：[1]福建医科大学微生物学教研室,福州350004 [2]福建医科大学附属第一医院消化内科

出　　处：《中国人兽共患病杂志》2000年第2期50-53,共4页Chinese Journal of Zoonoses

基　　金：福建省自然基金!95A003

摘　　要：目的指示球形幽门螺杆菌致细胞空泡变性毒力的变异情况。方法采用延期培养和加亚抑菌浓度抗生素，对细胞毒相关蛋白基因阳性（cagA＋），和细胞空泡毒素基因阳性（vacA＋）的高毒株幽门螺杆菌（Hp）进行球形诱变，进而检测该Hp至Hela细胞空泡变性的毒力，并用SDS-PAGE、PCR及PCR-SSCP技术，分析球形Hp蛋白产物及决定细胞空泡变性的cagA和vacA基因的变异情况。结果与结论球形Hp致细胞空泡毒力减弱，74KD以上的蛋白含量减少，特别是125KD条带尤为明显。259bp的vacA基因片段和349bpcagA基因片段未发生缺失，但两种方法诱变的球形HpvacA基因均存在点突变。Aim To reval the virulence variation related to vacuolating cytotoxicity of coccoid Helicobacter pylori.Methods The coccoid Helicobacter pylri, which was transformed from the bacterium with cagA gene and vacA gene by means of prolonged and antibiotics with subinhibitory conentaion, was detected the vacuolating cytotxin to Hela calls, analysed the differences of protein between the verity strain and its prototype by using SDS-PAGE, studied mutation of the vacA gene and cagA gene by means of PCR and PCR-SSCP Results and Conclusions.The vacuolating cytotoxicity,the proteins proteins with molecular weights above 74KD, especially 125KD, were decreased, no deletion was found in 259bp amplification fragments from vacA gene and 349bp amplification fragments from cagA gene but point mutation was existed in vacA gene of coccoid Hp.

关 键 词：球形幽门螺杆菌 细胞空泡毒素 毒力 基因突变 

分 类 号：R378.99[医药卫生—病原生物学]