三氧化二砷对大肠癌细胞的生长抑制作用  

Proliferation Inhibition Induced by Arsenic Trioxide on Colonic Neoplasms Cell Line SW620

作　　者：姚和瑞[1] 谢德荣[1] 詹俊[2] 陈岱佳[1] 向燕群[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院肿瘤科,广州510120 [2]中山医科大学孙逸仙纪念医院内科,广州510120

出　　处：《现代临床医学生物工程学杂志》2000年第1期18-19,共2页Journal of Modern Clinical Medical Bioengineering

摘　　要：目的观察三氧化二砷（ＡＳ_２Ｏ_３）对大肠癌细胞株ＳＷ６２０的生长抑制作用并初步探讨其机制．方法大肠癌细胞株ＳＷ６２０用不同浓度ＡＳ_２Ｏ_３处理．①ＭＴＴ法观察不同浓度ＡＳ_２Ｏ_３作用不同时间后对ＳＷ６２０细胞生长的影响；②增殖细胞核抗原（ＰＣＮＡ）单抗ＬＳＡＢ免疫组化染色－流式细胞仪检测 ＰＣＮＡ阳性细胞百分比．结果①ＡＳ_２Ｏ_３明显抑制大肠癌细胞株ＳＷ６２０细胞增殖，其作用随ＡＳ_２Ｏ_３浓度增加和作用时间延长而增加；②随ＡＳ_２Ｏ_３浓度增加，ＰＣＮＡ阳性细胞百分比逐渐下降（ｐ＜０． ０５）结论二氧化二砷可明显抑制大肠癌细胞株 ＳＷ６２０的生长，调控 ＤＮＡ合成及细胞增殖的 ＰＣＮＡ蛋白合成降低可能是 ＡＳ_２Ｏ_３的作用机制之一．Objective To observe the effect of proliferation inhibition of Arsenic Trioxide (AS_2O_3) on colonic neoplasms cell line SW620 and its mechanism. Methods Colonic neoplasms cell line SW620 was treated with different concentration AS_2O_3. MTT assay was used to observe the proliferation status. Immunohis tochemistry staining method and flow cytometry were used to measure the percentage of proliferating cell nuclear antingen (PCNA) positive cell. Results AS_2O_3 could significantly inhibit the growth of SW620 cell, and this effect was dose and time dependent. The percentage of PCNA positive cell was decreased gradually when concentration of AS_2O_3 was increased. Conclusions Arsenic Trioxide could inhibit the proliferation activity of colonic neoplasms cell line SW620. Reduction of PCNA which was related to the regulation Of DNA synthesis and cell proliferation might be the mechanism of this proliferation inhibition.

关 键 词：大肠肿瘤 三氧化二砷 增殖细胞核抗原 

分 类 号：R735.3[医药卫生—肿瘤]