慢性乙型肝炎重叠HGV感染对HBV复制的影响  

Influence of Hepatitis G Virus Superiufection upon Hepatitis B Virus Replication

作　　者：蔡卫平[1] 周颖[1] 石裕明[1] 

机构地区：[1]广州市第八人民医院,广州510060

出　　处：《现代临床医学生物工程学杂志》2000年第1期20-21,共2页Journal of Modern Clinical Medical Bioengineering

摘　　要：目的观察慢性乙型肝炎重叠ＨＧＶ感染对ＨＢＶ复制的影响．方法采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）检测患者血清ＨＢＶ－ＤＮＡ．ＨＢＶ－ＤＮＡ定量采用荧光信号引物能量转换法．以单纯慢性乙型肝炎患者作对照研究．结果２３例ＨＧＶ重叠感染的慢性乙型肝炎患者 ＨＢＶ－ＤＮＡ、ＨＢｅＡｇ和抗－ＨＢｅ的阳性率分别为５６．５％、１７．４％和６０．９％而３４例单纯慢性乙型肝炎患者三项指标的阳性率分别为９１．２％、８５．３％和１１．８％．定量分析显示，前组ＨＢＶ－ＤＮＡ量为２．０２、后组为４．１２．经统计学处理，两组各项指标均有显著性差异．结论慢性乙型肝炎重叠ＨＧＶ感染可能会抑制ＨＢＶ的复制．Objective To obesrve the influence of hepatitis G virus (HGV) superinfection upon hepatitis B virus (HBV) replication. Methods HGV-RNA in sera was detected by revers transcription polymerase chain reaction (RT-PCR). HBV-DNA was tested by PCR. HBV-DNA was quatitated by quantitative PCR assay which is based on Amplisenor assay. Results The positve rate of HBV-DNA, HBeAg and Anti-HBe of patients infected by HBV with HGV superinfection were 56.5%, 17.4% and 60.9% respectively. Whereas positive rates of patients with HBV infection alone were 91. 2%, 85.3% and 11.8% respectivly. The quantitative PCR analysis showed that the concentration of HBV-DNA in former group was significantly lower (2.02) than that in latter group (4.12) (P<0.05). Conclusions The superinfection of HGV in Hepatitis B patient could inhibit the replication of HBV.

关 键 词：慢性乙型肝炎 HGV HBV 重叠感染 病毒复制 

分 类 号：R575.1[医药卫生—消化系统]