烟酸对实验性动脉粥样硬化兔脂肪组织和细胞Caveolin-1表达的影响  

Effect of Niacin on Caveolin-1 Expression in Adipocytes and Adipose tissue of Experimental Atherosclerosis Rabbits

作　　者：文伟明[1] 陈秀林[2] 黄琛[3] 徐戈[1] 李志乐[1] 雷敏[1] 欧婷[1] 王利兵[1] 

机构地区：[1]广西医科大学第一附属医院心内科,广西百色市533000 [2]广西百色市人民医院内分泌科,广西百色市533000 [3]广西医科大学第一附属医院干部保健科,广西南宁市530021

出　　处：《中国动脉硬化杂志》2012年第11期985-989,共5页Chinese Journal of Arteriosclerosis

基　　金：广西青年科学基金资助(桂科青0832049)

摘　　要：目的探讨烟酸对实验性动脉粥样硬化兔脂肪组织和细胞小凹蛋白1(Caveolin-1)表达的影响。方法16只健康雄性新西兰大白兔给予高脂饮食8周,随机分为高脂组(继续饲以高脂饲料6周,n=8)和烟酸组[在高脂饮食基础上给予烟酸200 mg/(kg.d),共6周,n=8],另选8只兔给予普通饮食14周作为正常对照组。实验前后测定血脂和体重,14周末处死动物,取腹股沟皮下脂肪组织采用实时荧光定量PCR检测Caveolin-1 mRNA表达。体外观察不同浓度烟酸(0、0.2、1.0和5.0 mmol/L)对兔脂肪细胞Caveolin-1 mRNA表达的影响。结果与正常对照组相比,高脂组总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平均明显升高,主动脉内膜厚度和斑块面积均显著增加,而脂肪组织Caveolin-1 mRNA表达明显降低;与高脂组相比,烟酸组总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平均明显降低,高密度脂蛋白胆固醇水平升高,且主动脉内膜厚度和斑块面积均显著缩小,脂肪组织Caveolin-1 mRNA表达升高。体外实验显示,烟酸可呈浓度依赖性地刺激脂肪细胞Caveolin-1 mRNA表达。结论烟酸可明显改善血脂谱及稳定动脉粥样斑块,可能与上调脂肪组织或细胞Caveolin-1 mRNA表达水平有关。Aim To investigate the effect of niacin on expression of Caveolin-1 in adipocytes and adipose tissue from experimental atherosclerosis rabbits. Methods 16 New Zealand white rabbits were fed with high fat diet for 8 weeks, and then randomly divided into two groups ： hyperlipidemia group （maintained on high fat diet for 6 weeks, n -- 8） and niacin treatment group （the same fat diet plus 200 mg/ （kg ~ d） niacin for 6 weeks, n = 8 ）. Rabbits in control group （n = 8） were fed with normal diet for 14 weeks. At the end of the study, all rabbits were sacrificed to detect the blood lipid changes and Caveolin-1 expression in adipose tissue by real-time quantitative PCR. The direct effect of niacin on Caveolin-1 mRNA expression in primary rabbit adipocytes was assayed. Results Compared with control group, rabbits fed with high fat diet showed higher serum levels of total cholesterol, triglyceride and low density lipoprotein choles- terol, more aorta endometrial thickness and plaque area, less Caveolin-1 expression. Compared with hyperlipidemia group, rabbits treated with niacin showed less serum levels of total cholesterol, triglyceride and low density lipoprotein cho- lesterol, less aorta endometrial thickness and plaque area and more Caveolin-1 expression. In vitro study, niacin dose-dependently stimulated Caveolin-1 expression in cultured adipocytes. Conclusions Niacin could improve the lipid profile and stabilize atheromatous plaque, which may be related to up-regulating the expression of Caveolin-1 in adipose tissue or adipocytes.

关 键 词：烟酸 动脉粥样硬化 小凹蛋白1 脂肪组织 

分 类 号：R363[医药卫生—病理学]