轻度认知功能障碍和阿尔茨海默病患者血小板α和β分泌酶活性的变化  

Platelet α and β secretase activities in mild cognitive impairment and Alzheimer＇ s disease

作　　者：杨红旗[1] 冯淑曼[1] 蒋秋焕[1] 马建军[1] 

机构地区：[1]河南省人民医院神经内科,郑州450003

出　　处：《中华神经科杂志》2012年第11期783-786,共4页Chinese Journal of Neurology

基　　金：河南省科技攻关计划项目资助（112102310684）

摘　　要：目的研究轻度认知功能障碍（MCI）和阿尔茨海默病（AD）患者血小板α和β分泌酶活性及α分泌酶的产物sAPPα含量的变化。方法对48例其他疾病老年对照者、42例MCI患者及40例AD患者分别进行神经功能评定，用荧光法测定各组患者血小板α和β分泌酶的活性，并用Western印迹检测血小板sAPPα含量。结果对照组、MCI组和AD组患者α分泌酶活性分别为100．0％±10．6％、78．2％±9．4％和61．8％±7．2％，MCI和AD组与对照组相比均降低，差异有统计学意义（F=22．935，P=0．001）；AD组与MCI组相比亦明显降低，差异有统计学意义。对照组、MCI组和AD组β分泌酶的活性分别为100．0％±11．2％、145．8％±12．7％和189．8％±14．2％；MCI组和AD组均较对照组升高，差异有统计学意义（F=16．368，P=0．001），AD组较MCI组高，差异有统计学意义。sAPPα的含量MCI组和AD组与对照组相比均明显降低；AD组与MCI组相比亦显著降低，差异有统计学意义。结论MCI组和AD组患者的α分泌酶活性及其产物sAPPα均较对照组降低，β分泌酶活性升高；α和β分泌酶活性改变可能在MCI和AD的发病过程中起着一定的作用，在MCI和AD的诊断中有一定的潜在价值。Objective To investigate platelet α and β secretase activities and the amounts of platelet soluble fragment of APP （sAPPct） produced by α-secretase in patients with mild cognitive impairment （MCI） and Alzheimer＇ s disease （ AD ） . Methods The neurological functions of 48 non- demented patients, 42 MCI and 40 AD patients were evaluated by neuropsychological examinations. The platelet αand β secretase activities and sAPPα production in each group were measured by fluorescence and Western blotting analysis respectively. Results The α secretase activities in non-demented, MCI and AD group were 100. 0% ± 10. 6%, 78.2% ± 9. 4% and 61.8% ± 7. 2% respectively. As compared with non- demented group, the α seeretase activities in MCI and AD group were decreased （ F = 22. 935, P = 0. 001 ）. The α secretase activity in AD group was significantly lower than MCI group. The β seeretase activities in non-demented, MCI and AD group were 100. 0% ± 11.2% , 145.8% ± 12. 7% and 189. 8% ± 14. 2% respectively. The β secretase activities in MCI and AD group were significantly higher than that in non- demented group （ F = 16. 368, P = 0. 001 ）. The β secretase activity in AD group was significantly decreased as compared with MCI group. The sAPPer amounts in MCI group and AD group were all decreased as compared with that in control group; the sAPPα amount in AD patients was significantly decreased as compared with that in MCI group. Conclusions The platelet α secretase activity and its production sAPPα in MCI and AD patients are decreased, while β secretase activity is increased, as compared with that in control group; the altered α and β secretase activities may participate in the pathogenesis of MCI and AD patients and may have diagnostic potential for them.

关 键 词：认知障碍 阿尔茨海默病 淀粉样前体蛋白分泌酶类 

分 类 号：R749.16[医药卫生—神经病学与精神病学]