长春西汀对大鼠脑缺血再灌注损伤炎性反应机制的影响  

The effect of vinpocetine on the inflammation of rats after cerebral ischemia-reperfusion as well as expression of nuclear factor-kappa B

作　　者：张侃[1] 王洪新[1] 闫玉仙[2] 唐江伟[1] 高路燕[1] 魏中平[1] 

机构地区：[1]天津医科大学附属第四中心医院神经内科,300140 [2]武警后勤学院中心实验室

出　　处：《中国神经免疫学和神经病学杂志》2012年第6期444-448,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：天津市卫生局资助课题(2011ky04)

摘　　要：目的探讨长春西汀对大鼠脑缺血再灌注损伤炎性反应机制的影响。方法将Wistar大鼠随机分为假手术组、脑缺血再灌注损伤组和长春西汀干预组,应用线栓法制备大脑中动脉闭塞2h后再灌注模型,分别于再灌注6h、24h、3d、7d采用2,3,5-氯化三苯基四氮唑(TTC)染色法检测脑梗死灶大小,采用干、湿重法检测脑组织含水量以评价脑水肿的程度,采用免疫组织化学和原位杂交的方法检测大鼠核转录因子(nuclear factor-kappa B,NF-κB)、肿瘤坏死因子α(TNF-α)的表达。结果与假手术组比较,模型组NF-κB的表达于再灌注6h即增高(P<0.05),TNF-α的表达于24h增高(P<0.05),3d时均达高峰(均P<0.05),7d时仍高于假手术组(均P<0.05);与模型组比较,长春西汀干预组于再灌注后24h、3d、7d时NF-κB和TNF-α表达降低。长春西汀干预组NF-κB各时点组内比较未见统计学差异(F=2.324,P>0.05)。与假手术组比较,模型组和长春西汀干预组脑组织水含量于脑缺血再灌注损伤6h即增高,3d时达到高峰(均P<0.05),7d时仍高于假手术组(均P<0.05)。结论长春西汀对大鼠脑缺血再灌注损伤炎性反应有明显的抑制作用。Objeetive To explore the effect of vinpocetine on the inflamation of rats after cerebral ischemia-reperfusion. Methods The wistar rats were randomly assigned to a sham-operation group, a cerebral ischemia-reperfusion group and a vinpocetine cerebral ischemia-reperfusion group. A model of middle cerebral artery occlusion for 2 h followed by reperfusion was induced in rats using the suture method. The infarct size was determined by 2, 3, 5-triphenyi terazoloride （TTC） staining at 6 h, 24 h, 3 d, and 7 d respectively after the reperfusion. Dry-wet weight method was used to measure brain water content and evaluate the extent of brain edema. The methods immunohistochemistry and in-situ hybridization were used to detect the expression of NF- κB and TNF-α. Results Compared with control group, NFκB level of ischemia-reperfusion group in ischemia brain tissue increased at 6 h （P〈0.05） after the reperfusion and TNF-α level increased at 24 h （P〈0.05）. Both of them reached the peak at day 3 （both P〈0.05）. After that, it was decreased gradually, but it was still higher than that in the sham-operation group at day 7 （both P〈0.05）. Compared with ischemia-reperfusion group, TNF-α decreased in vinpocetine-treated group since 24 h and the expression of them were lower at 3 d （both P〈0.05）. NF-κB of vinpocetine-treated group had no significant difference in comparison within group （F =2. 324, P 〈 0.05）. At different time points, cerebral ischemia-reperfusion group compared with control group, the infarct volume and brain edema have great difference. Conclusions NF-κB and TNF-α is associated with the changes of brain edema and infact volume and vinpocetine decreases the expression of NF-κB, TNF-α and inhibite inflamating response after cerebral ischemia-reperfusion.

关 键 词：长春西汀 再灌注损伤 炎症 NF—κB 肿瘤坏死因子-Α 

分 类 号：R743.3[医药卫生—神经病学与精神病学]