重组胸腺素α_1 pMAL-C2x-Tα_1/TB1工程菌三角摇瓶培养条件的优化  

Optimizing the cultivation condition of engineering bacterial E. coli pMAL-C2x-Tα1/TB1 with the flask-shaking method

作　　者：刘中禄[1] 陶翠兰[1] 莘旭妮[2] 李树民[2] 

机构地区：[1]第三军医大学大坪医院野战外科研究所,400042 [2]中国人民解放军军事医学科学院军事兽医研究所

出　　处：《实用医技杂志》2012年第11期1128-1131,共4页Journal of Practical Medical Techniques

摘　　要：目的对重组胸腺素α1pMAL-C2x-Tα1/TB1工程菌的培养条件进行优化。方法在摇瓶培养条件下探讨工程菌的生长和表达规律,对pMAL-C2x-Tα1/TB1菌种的培养基装量、接种量、诱导剂异丙基硫化半乳糖苷(IPTG)加入时间和浓度、诱导时间进行了实验和优化。结果由摇瓶培养获得的数据表明pMAL-C2x-Tα1/TB1最优培养条件为:10%接种量、100 mL LB培养基、37℃培养,接种后3 h,入终浓度为0.75 mmol/L的IPTG,诱导4 h后目的蛋白表达量最高。结论优化的培养条件为菌种的发酵工艺奠定了基础。Objective To optimize the cultivation condition for maximizing the productivity of recombinant thymosin alpha1 protein from engineering bacterial E. coli pMAL-C2x-Tαl/TB1. Methods The best cultivation and induction condition to yield the highest production were explored with the flask-shaking method. Results The data obtained from flask-shaking showed that the best expression could be achieved by cell growth, The best cultivation condition started from 10% inoculation, in 100 mL LB medium at 37 ℃ for 3 hours, followed by additional 4 hours induction with 0.75 mmol/L IPTG. Conclusion The optimal production technic of recombinant thymosin alpha 1 was established to laid a foundation to produce recombinant thymosin alpha 1 on a large scale fermentation.

关 键 词：胸腺素 发酵 条件优化 

分 类 号：R82[医药卫生—临床医学]