白及多糖琥珀酸酯的抗氧化活性研究  

Study on the antioxidant activities of succinated glucomannans from Bletilla striata

作　　者：王虎成[1] 孙燕[1] 俞樱露[1] 刘江云[1] 蔡培烈[1] 杨世林[1] 

机构地区：[1]苏州大学医学部药学院,江苏苏州215123

出　　处：《苏州大学学报（医学版）》2012年第5期597-599,618,共4页Suzhou University Journal of Medical Science

基　　金：国家"十一五"科技支撑计划项目(2009BA174B02);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：目的研究琥珀酸酯改性白及多糖的抗氧化活性。方法分别采用Fenton法和邻苯三酚自氧化法测定不同取代度(DS)白及多糖琥珀酸酯BT-2(DS=0.38)、BT-3(DS=0.69)、BT-4(DS=0.85)对OH.和O2-.的抗氧化活性。结果白及多糖琥珀酸酯对OH.的半数清除率浓度(mg/ml)依次为1.48±0.01(BT-4)、2.65±0.01(BT-3)、2.79±0.02(BT-2);对O2-.的半数清除率浓度(mg/ml)依次为1.38±0.06(BT-4)、6.44±0.02(BT-3)、21.33±0.13(BT-2)。结果显示,不同取代度白及多糖琥珀酸酯对OH.和O2-.的清除能力大小依次为BT-4>BT-3>BT-2(P<0.05)结论白及多糖琥珀酸酯的抗OH.和O2-.活性随取代度增大而显著增强。Objective To study the antioxidant activities of succinated glucomannan from Bletilla striata.Methods Antioxidant activities of succinated glucomannan with different degree of dubstitution（DS） including BT-2（DS=0.38）,BT-3（DS=0.69） and BT-4（DS=0.85） against OH·and O-2·were measured by Fenton reaction and autoxibation of pyrogallic acid.Results The IC50（mg/ml） of succinated glucomannans against OH· was measured to be 1.48±0.01（BT-4）,2.65±0.01（BT-3）,2.79±0.02（BT-2）;while their IC50（mg/ml） against O-2·was determined to be 1.38±0.06（BT-4）,6.44±0.02（BT-3）,21.33±0.13（BT-2）,respectively.Conclusion The antioxidant activities of succinated glucomannans against OH·and O-2· were increased apparently with increasing DS.

关 键 词：白及 白及多糖琥珀酸酯 取代度 抗氧化活性 

分 类 号：R961.1[医药卫生—药理学]