硼替佐米对K562细胞株DNA甲基转移酶表达的影响  

The effects of bortezomib on the expression of DNA methyltransferase in K562 cells

作　　者：吴圣豪[1] 郑翠苹[1] 徐杰[1] 

机构地区：[1]浙江省温州市第二人民医院血液化疗科,325000

出　　处：《中国药物与临床》2012年第11期1405-1407,共3页Chinese Remedies & Clinics

基　　金：浙江省温州市科技计划项目(Y20100070)

摘　　要：目的探讨蛋白酶体抑制剂硼替佐米(bortezomib,商品名:万珂)对K562细胞株DNA甲基转移酶(DNMTs)表达、细胞凋亡的影响。方法常规体外培养白血病K562细胞株,随机将处于对数生长期的细胞分为12、24、36h3个作用时间组,予以不同浓度的硼替佐米:0,6,20,60nmol/L,蛋白印迹法检测胞内DNMT1的表达,流式细胞术检测细胞凋亡。结果与阴性对照组相比,硼替佐米可显著抑制DNMT1表达。硼替佐米作用于细胞12、24、36h后细胞凋亡率逐渐增加,60nmol/L硼替佐米作用36h后细胞凋亡率为(61.68±3.20)%。结论硼替佐米抑制K562细胞株DNMT1表达,诱导细胞凋亡,该作用呈浓度依赖性。Objective To investigate the effects of bortezomib on the expression and apoptosis in DNA methyltransferase （DNMTs） in K562 cells. Methods The K562 cells of leukemia were cultured conventionally in vitro, among which the cells in logarithmic phase were randomized into 12 h, 24 h and 36 h these three action time groups and given bortezomib of different concentrations： 0, 6, 20, 60 nmol/L. The expression of DNMT1 was detected by Western blotting and the cell apoptosis by flow cytometry. Results Compared with the negative control group, bortezomib could significantly inhibit the expression of DNMT1. The cell apoptosis rate was gradually increased at 12 h, 24 h and 36 h after treatment of bortezomib. Notably, the apoptosis rate was （61.68±3.20）% at 36 h after treatment of 60 nmol/L bortezomib. Conclusion Bortezomib could inhibit the expression of DNMT1 in K562 cells and induce the cell apoptosis. And the effects were in concentration-dependent pattern.

关 键 词：白血病 硼替佐米 DNA甲基转移酶 

分 类 号：R733.7[医药卫生—肿瘤]