雌孕激素对ALI大鼠Ⅱ型肺泡上皮细胞ENaC表达的影响  

Influence of estradiol and progestogen in type Ⅱ alveolar ENaC in rats with acute lung injury

作　　者：罗玲[1] 王导新[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科,重庆400010

出　　处：《吉林大学学报（医学版）》2012年第5期836-840,F0002,共6页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30971303)

摘　　要：目的:研究雌孕激素对急性肺损伤(ALI)大鼠Ⅱ型肺泡上皮细胞钠通道(ENaC)表达的影响,探讨雌孕激素促进肺泡内液体清除的机制。方法:雌性未成年SD大鼠40只,随机分为正常对照组、ALI模型组、雌孕激素联合治疗A组和雌孕激素联合治疗B组,正常对照组颈静脉注入生理盐水(8mL.kg-1),后3组经颈静脉注入油酸(0.1mL.kg-1)建立ALI模型,其中雌孕激素联合A、B组分别在油酸注入前12和36h皮下注射不同比例的雌孕激素混合液0.1mL(A组1∶1 000,B组1∶200)。建模后6h处死大鼠,测定肺湿重/干重比值,计算肺系数,观察肺组织病理改变,测定肺组织匀浆中α-ENaC、β-ENaC和γ-ENaC mRNA及蛋白的表达水平。结果:①与正常对照组比较,ALI模型组肺湿重/干重比值及肺系数增加,差异有统计学意义(P<0.01);与ALI模型组比较,雌孕激素联合治疗组肺湿重/干重比值及肺系数下降,以A组明显,差异有统计学意义(P<0.05)。②ALI模型组大鼠出现明显肺水肿、出血及炎性细胞浸润;与模型组比较,联合治疗组大鼠肺水肿、出血及炎性细胞浸润较轻,A组稍明显。③与正常对照组比较,ALI组α-ENaC和γ-ENaC mRNA及蛋白表达水平明显降低,差异有统计学意义(P<0.01);与ALI模型组比较,联合治疗组α-ENaC和γ-ENaC mRNA及蛋白表达水平有增加,以A组增加明显,差异有统计学意义(P<0.05)。结论:雌孕激素可以通过增加ALI大鼠Ⅱ型肺泡上皮细胞α-ENaC和γ-ENaC mRNA及蛋白的表达水平,促进肺泡内液体的清除。Objective To study the influence of 17β-estradiol and progestogen in type Ⅱalveolar epithelial Na＋ channel（ENaC）in rats with acute lung injury（ALI） and explore the mechanism of estradiol and progestogen in promoting the clearance of alveolar fluid.Methods 40 immature female SD rats were randomly assigned into 4 groups： normal control group（injected with 8 mL·kg-1 saline via venous）,ALI model group,17β-estradiol and progestogen combination A and B group.The rats in last three group were injected with 0.1 mL·kg-1 oleic acid via venous to establish ALI model.The rats in combination A and B groups were injected subcutaneously with 17β-estradiol and progestogen mixed liquor 0.1 mL in different ratios（A group 1∶1 000,B group 1∶200） at 12 and 36 h before oleic acid injection.All the rats were sacrificed at 6 h after oleic acid injection.The wet/dry ratio and the lung coefficient were determined,the pathological changes of lung tissues were observed,and the expressions of α-ENaC,β-ENaC and γ-ENaC mRNA and protein were determined.Results ① Compared with normal control groups,the wet/dry ratio and the lung coefficient in ALI model group were increased significantly（P0.01）;in combination A and B groups,the wet/dry ratios and the lung coefficients were decreased compared with model group,especially in A group（P0.05）.②Obvious pulmonary edema,bleeding and inflammatory cell infiltration were observed in ALI group.However,the extent of pulmonary edema,bleeding and inflammatory cell infiltration were decreased markedly in combination A and B groups,especially in A groups.③ Compared with normal control group,the expressions of α-ENaC and γ-ENaC mRNA and protein were decreased in ALI model group（P0.01）;compared with ALI group,they were increased significantly in combination A and B groups,especially in A group（P0.05）.Conclusion 17β-estradiol and progestogen can increase the expressions of type Ⅱ alveolar epithelial α-ENaC and γ-ENaC mRNA and protein in rats with ALI,and

关 键 词：雌激素 孕激素 钠通道 急性肺损伤 急性呼吸窘迫综合征 

分 类 号：R363[医药卫生—病理学]