水溶性壳聚糖对腹主动脉球囊损伤大鼠血管狭窄的抑制作用  

Inhibitory effects of water-soluble chitosan on angiostenosis of aorta ventralis of rats with balloon injury

作　　者：王朋[1] 费瑜[1] 吕丽丽[1] 张帆[1] 杨明[2] 王洋[2] 邓扬[3] 

机构地区：[1]吉林大学第二医院心血管内科,吉林长春130041 [2]吉林大学白求恩医学院细胞生物系,吉林长春130021 [3]吉林省人民医院急诊科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2012年第5期880-884,I0001,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅自然科学基金资助课题(201115202);吉林省科技厅科技发展计划项目资助课题(20100920)

摘　　要：目的:探讨水溶性壳聚糖(WSC)在血管狭窄方面的作用,阐明WSC对大鼠腹主动脉球囊损伤的影响,为开发防治血管狭窄的高效低不良反应药物提供实验依据。方法:75只雄性SD大鼠随机分5组:50、100和200mg.kg-1的WSC组、模型组和对照组(假手术组),每组15只。采用球囊损伤大鼠腹主动脉内膜方法制备血管狭窄模型,对照组仅分离结扎右髂总动脉。造模后WSC各组经大鼠尾静脉分别注射50、100和200mg.kg-1浓度的WSC;模型组和对照组注射生理盐水,每次0.4mL,每天1次。每组按7、14和21d3个时点随机选取5只大鼠处死,常规HE染色制作病理切片,观察各组大鼠血管损伤形态,计算相对管腔面积,比较WSC对血管狭窄的抑制作用。结果:普通光学显微镜观察,对照组大鼠腹主动脉结构完整,中膜血管平滑肌细胞排列整齐。模型组大鼠在第7天时腹主动脉血管内膜增生明显;第14天时可见内膜、中膜明显增厚,管壁局限性隆起;第21天内膜增厚,以平滑肌细胞为主,中膜细胞排列紊乱,有假腔形成。与模型组比较,实验组第7天时内膜增生明显减轻,以100和200mg.kg-1 WSC组为最好;第14天时可见内膜、中膜增厚均不明显,但200mg.kg-1 WSC组仍可见大量排列紊乱的血管平滑肌细胞;第21天时各浓度的WSC组均表现正常,其中以200mg.kg-1 WSC组更为明显。管腔相对面积比较,对照组大鼠管腔面积随术后时间变化不明显(P>0.05);模型组大鼠管腔面积在第7、14和21天时均减小,与对照组比较差异有统计学意义(P<0.01)。50mg.kg-1 WSC组大鼠在第14天和第21天时,管腔面积有一定的增加,但与对照组比较差异有统计学意义(P<0.01);100mg.kg-1 WSC组大鼠的管腔面积明显大于50mg.kg-1 WSC组,其中在第14天和第21天时的管腔面积与对照组比较差异有统计学意义(P<0.05);200mg.kg-1 WSC组的管腔面积在多于14d时为最大,与对照组相比较差异无统计学意义(P>0.05)。各实验Objective To explore the effects of water-soluble chitosan（WSC）on angiostenosis and balloon injury of rat aorta ventralis,and to provide the basis for the development of high efficiency and low side-effect drug on prevention of angiostenosis.Methods Seventy-five SD male rats were randomly divided into five groups： 50,100 and 200 mg·kg-1 WSC groups,model group and control group（n=15）.The angiostenosis animal model was established by balloon to injure rat aorta ventralis.The right common iliac artery was ligated for the rats in control group.After establishing the animal model of angiostenosis,the WSC of 50,100 and 200 mg·kg-1 concentrations was respectively injected into vena caudalis of the rats in WSC groups,and normal saline were injected into vena caudalis of the rats in model group and control group,0.4 mL every time and 1 time every day.At the 7th,14th and 21st days after the operation,five rats in each group were randomly sacrificed,aorta ventralis were obtained and their pathological sections of HE dyeing were prepared.The pathological changes of blood vessel in each rat were observed with microscope and the ratios of lumina area to extima area at different time were counted.The inhibitory effects of WSC on angiostenosis were compared among five groups.Results Under microscope,a complete structure of aorta ventralis was observed and smooth muscle cells of tunica media were arranged in order in control group.In the aorta ventralis of the rats in model group,obvious proliferation of intima was observed at the 7th day after the operation,thickening tunica intima,thickening tunica media and localized proliferation of vessel wall were also observed at the 14th day after the operation,and many smooth muscle cells in thickening tunica intima were discovered,and smooth muscle cells of tunica media were arranged disorder and false lumen was formed at the 21st day after the operation.Compared with model group,at the 7th day after the operation,the proliferation of tunica intima was obviously lessen in ex

关 键 词：水溶性壳聚糖 大鼠 Sprague-Dawley 腹主动脉 球囊损伤 血管狭窄 

分 类 号：R363[医药卫生—病理学]