拟南芥中eIF-5A基因的克隆、载体构建及转化  

Gene Cloning, Carrier Construction and Transformation of eIF -5 A in Arabidopsis thaliana

作　　者：赵金海[1] 段楠[1] 丁锐 宫禹 王雷 李瑶 

机构地区：[1]黑龙江省科学院高技术研究院,哈尔滨150001 [2]黑龙江省北安市红星农场,黑龙江北安164000

出　　处：《黑龙江科学》2012年第5期70-75,共6页Heilongjiang Science

基　　金：黑龙江省科学院青年基金

摘　　要：试验首先利用eIF-5A基因表达的上调或下调来影响拟南芥性状，进一步研究通过调控DHS酶的表达上调或下调，影响调控eIF-5A的表达，进而影响拟南芥性状。与普通条件下种植的拟南芥对比，含有eIF-5A获得的植株，转化率约为37％，产量为原野生植株的124％，抗病及抗逆性都比原拟南芥高50％以上。This paper first affected the arabidopsis properties by the eIF- 5 A rise and fall of gene expression, and affected and regulated elf -5 A expression through the regulation of DHS enzyme rise and fall,further affected arabidopsis properties. Com- pared with arabidopsis grown on common conditions, conversion rate of plants containing eIF- 5 A is about 37% and the yield is 124% of the wild plants, Disease resistance is 50% higher than the common arabidopsis.

关 键 词：拟南芥eIF-5A 基因 载体构建 基因克隆 

分 类 号：Q943.2[生物学—植物学]