乙型肝炎病毒X基因对HepG2肝癌细胞生物学行为的影响及其机制  

Effects and mechanism of hepatitis B virus X gene on the biological behaviors of human hepatocarcinoma cell line HepG2

作　　者：刘瑶[1] 黄文峰[1] 黄才斌[1] 

机构地区：[1]赣南医学院第一附属医院消化内科,341000

出　　处：《实用医学杂志》2012年第22期3695-3697,共3页The Journal of Practical Medicine

基　　金：江西省教育厅科技项目(编号:GJJ11566);江西省自然科学基金项目(编号:2010GQY0036)

摘　　要：目的:研究乙型肝炎病毒X基因(HBX)对HepG2细胞生物学行为的影响及机制。方法:HepG2瞬时转染HBX真核表达载体pcDNA3.1-HBX(HepG2/HBX),转染pcDNA3.1的HepG2细胞和未转染任何质粒的HepG2细胞分别作为空白对照组和阴性对照组。转染后48h收集细胞,流式细胞仪分析细胞凋亡率的变化;transwell检测细胞侵袭能力;RT-PCR检测PEG10mRNA表达量的变化。结果:实验组细胞凋亡率为(4.8±1.8)%,空白和阴性对照组细胞凋亡率分别为(12.9±1.4)%和(11.7±2.2)%,P<0.05。实验组细胞体外侵袭力增强(P<0.05),PEG10基因的表达水平明显上调(P<0.05)。结论:HBX通过上调PEG10的表达,抑制HepG2细胞凋亡并增强其体外侵袭能力。Objective To investigate the effect and mechanism of hepatitis B virus X gene on biological behaviors of human hepatocarcinoma eel1 line HepG2. Method HBX gene eukaryotic expression vector peDNA3.1- HBX was transiently transfected into HepG2 cells. HepG2 transfected with pcDNA3.1and HepG2 were served as blank control group and negative control group, respectively. Cells were harvested at 48h after transfeetion. Cells apoptosis rates were assessed by flow cytometry with annexin-FITC/PI staining. The changes of invasion ability of HepG2 cells were investigated by transwell invasion assay. The mRNA expression of PEG10 was detected by using RT-PCR. Results FCM analysis showed that the apoptosis rate of HepG2/HBX cells was （4.8 ±1.8）%, blank control and negative control group were （12.9± 1.4） % and （11.7±2.2） %, respectively. Transwell assay showed that the invasion ability of HepG2/HBX cells was enhanced （P 〈 0.05）. The RT-PCR result showed that the expression of PEG10 gene was up-regulated in HepG2/HBX cells （P 〈 0.05）. Conclusion HBX inhibit HepG2 cell apoptosis and enhance invasion ability mainly by up-regulating the expression of PEG10.

关 键 词：PEG10 乙型肝炎病毒X基因 HEPG2细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]