基因芯片分析IFRD1表达沉默后肝癌细胞周期相关基因表达的变化  

Gene expression changes of hepatocellular carcinoma cell cycle after IFRD1 silencing by Microarray analysis

作　　者：胡卫[1] 方肇勤[2] 梁超[2] 管冬元[2] 吴中华[2] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]上海中医药大学基础医学院,上海201203

出　　处：《中国中医基础医学杂志》2012年第11期1207-1210,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(30672574;30973703)

摘　　要：目的:分析IFRD1基因表达下调后人肝癌细胞周期中基因的改变,探讨该基因影响肝癌细胞增殖的机制。方法:采用RNA干扰技术下调目的基因表达,再用基因芯片检测人肝癌细胞表达谱的改变,重点分析细胞周期通路中基因的表达变化。结果:IFRD1干扰后,细胞周期蛋白和CDK表达上调,而抑制因子CKI以下调为主;G1/S期检测点p21、p57表达均下调;S期MCM蛋白复合体以下调为主,ORC表达均下调,而CDC6和CDC45表达也下调,提示染色体复制受阻。结论:IFRD1表达沉默后,细胞生长活跃,G1-S期转换顺利,而S期DNA合成受到抑制,有丝分裂检测点受阻,最终导致细胞增殖分裂受限。Objective ：Analyze the changes of genes in cell cycle after IFRD1 gene was down regulated by RNA inference,to explore mechanism of its effect on proliferation of liver cancer cells.Methods：RNA inference technology depressed the expression of IFRD1 gene,then gene chip（Affymetrix HU133 plus 2.0） was applied to investigate the alteration of gene expression profiles.Bioinformatics was used to analyze data,special focus on cell cycle related genes.Results： After RNA inference,Microarray assay revealed that cyclin and cyclin-dependent kinase were up-regulated,the inhibitor CKI mainly downregulated;G1/S checkpoint p21、p57 were both downregulated;DNA synthesis onset related molecules were depressed in S phase.Conclusion：After the silence of IFRD1 gene,the result revealed that cell growed vigorously,G1/S transition was smoothly,but DNA synthesis in S phase was depressed,it ultimately leaded to slow cell proliferation.

关 键 词：IFRD1 细胞周期 基因芯片 RNA干扰 

分 类 号：R735.7[医药卫生—肿瘤]