灵芝菌丝体多糖体外抗氧化活性研究  

Study on anti-oxidation activity of polysaccharide extracted from mycelia of Ganoderma lucidum

作　　者：连瑞丽[1] 李宇伟[1] 李存法[1] 陈忠杰[1] 班新河 李九英 

机构地区：[1]郑州牧业工程高等专科学校药物工程系,河南郑州450011 [2]驻马店农业科学院,河南驻马店463000

出　　处：《广东农业科学》2012年第19期93-94,101,共3页Guangdong Agricultural Sciences

基　　金：河南省教育厅科技攻关计划项目(2011A230014;2009B210025)

摘　　要：对灵芝菌丝体的多糖含量进行了测定,得多糖含量为3.84%。运用DEAE Sephacel柱层析技术从菌丝体中纯化出M1、M2和M3等3种多糖组分,分别占菌丝体多糖总量的47.19%、31.18%、11.02%。体外抗氧化活性试验结果显示,M1对羟基自由基(.OH)和超氧阴离子自由基(O2-.)的清除能力最强,M2次之,M3最弱。The polysaccharide content of mycelia from Ganoderma lucidum was determined and its value was 3.84%. The methods of DEAE Sephacel column chromatography was employed to purify the raw polysaccharide of mycelia. The results showed that there were three kinds of fractions marked M1, M2 and M3 in raw polysaccharide of mycelia, contents of which were 47.19%,31.18% and 11.02% respectively. In the anti-oxidantaction experments of scavenging hydroxylr adical （·OH） and superroxide anion radical （O2-.）, M 1 had the strongest power, M2 was second and M3 was the weakest.

关 键 词：灵芝 菌丝体 多糖 抗氧化 

分 类 号：R285[医药卫生—中药学]