IFN-α联合5-FU对肝癌大鼠的作用  

Curative Effect of Interferon Alpha Combined with 5-FU on Apoptosis of Liver Cancer Cells

作　　者：周勇慧[1] 王家宏[1] 

机构地区：[1]包头医学院第一附属医院,内蒙古包头014010

出　　处：《包头医学院学报》2012年第5期14-16,共3页Journal of Baotou Medical College

摘　　要：目的:探讨α干扰素(IFN-α)联合5-FU对肝癌大鼠的作用。方法:将大鼠随机分为空白对照组,模型组,5-FU组,IFN-α联合5-FU组;将传代后肝癌腹水注入大鼠肝脏,建立大鼠肝癌模型。IFN-α联合5-FU组大鼠,IFN-α皮下注射3×105U/d、5-FU 30 mg/kg腹腔注射;5-FU组大鼠,5-FU腹腔注射30 mg/kg;空白对照组及模型组腹腔注射同等剂量生理盐水;连续35 d,然后取血、处死,取肝脏,观察肿瘤生长情况以及血清Cyclin D1及NF-κB的表达。结果:与空白对照组比较,模型组肿瘤生长增快,血清Cyclin D1与NF-κB表达明显增高,差异有统计学意义;与模型组比较,5-FU组与IFN-α联合5-FU组大鼠肿瘤生长减慢、Cyclin D1与NF-κB表达降低,差异均有统计学意义;与5-FU组相比,联合用药组肿瘤生长减慢,Cyclin D1与NF-κB表达降低,差异也均有统计学意义。结论:与单独抗癌药物相比,IFN-α与5-FU联合使用可有效降低肝癌大鼠肿瘤生长速度以及血清Cyclin1及NF-κB的表达。Objective： To observe the curative effect of Interferon alpha（IFN-α） combined with 5-FU on apoptosis of Liver cancer cells by the establishment of animal model.Methods： The rats were randomly divided into the blank control group,model group,5-FU group,5-FU group of alpha interferon.A model of hepatocellular carcinoma was established by injecting subcultured ascites into rat liver.The rats in 5-FU group of interferon alpha had subcutaneous IFN-αinjection of 3×105 U/d,those in 5-FU group had abdominal injection of 30 mg/kg and those in the blank control and model group had same dose abdominal injection of physiological saline.After 35 days of successive injection,from these rats the blood and liver were taken to observe carcinoma growth state,and the expression of serum CyclinD1 and NF-κB.Results： Compared with that in the blank control group,the tumor growth rate increased significantly and the expression of serum CyclinD1 and NF-κB went up in model group.Compared with that in the model group,tumor growth rate was significantly lowered and the expression of serum CyclinD1 and NF-κB dropped in 5-FU group and 5-FU group of alpha interferon.Compared with that in 5-FU group,tumor growth was significantly slower and the expression of serum CyclinD1 and NF-KB was even lower in 5-FU group of alpha interferon.Conclusion： Compared with anticancer drugs singly administered,Combined administration of IFN-αand 5-FU can effectively lower the tumor growth rate and Cyclin1and KF-KB expression in rats with liver cancer.

关 键 词：Α干扰素 5-FU 联合用药 肝癌 疗效 

分 类 号：R735.7[医药卫生—肿瘤]