高尔基体α-甘露糖苷酶Ⅱ、E-cadherin和α-catenin在卵巢上皮肿瘤组织和不同转移能力的卵巢癌细胞中的表达  

The expressions of GMⅡ, E-cadherin and α-catenin in ovarian epithelial tumor tissues and ovarian cancer cells with di erent abilities of metastasis

作　　者：卢玉娟[1] 罗祎[2] 杨雅莹[1] 易永芬[1] 

机构地区：[1]重庆医科大学病理教研室,分子医学与肿瘤研究中心,重庆400016 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《肿瘤》2012年第11期899-906,共8页Tumor

基　　金：国家自然科学基金资助项目(编号:30672431);高等学校博士学科点专项科研基金(编号:20060631006);重庆市自然科学基金资助项目(编号:2010BB5361)

摘　　要：目的:检测高尔基体α-甘露糖苷酶Ⅱ(Golgiα-mannosidaseⅡ,GMⅡ)、E-cadherin和α-catenin在不同卵巢上皮肿瘤组织和不同转移能力的卵巢癌细胞中的表达。方法:运用免疫组织化学法检测46例卵巢上皮恶性肿瘤(其中有大网膜或淋巴结转移者27例)、15例卵巢上皮交界性肿瘤及12例卵巢上皮良性肿瘤组织中GMⅡ、E-cadherin和α-catenin的表达情况。体外培养卵巢癌A2780、SKOV-3和HO-8910细胞,分别应用RT-PCR、免疫荧光和蛋白质印迹法检测GMⅡ、E-cadherin和α-catenin mRNA和蛋白的表达情况。结果:在卵巢上皮恶性肿瘤中GMⅡ的阳性表达率(87%)和E-cadherin、α-catenin的异常表达率(80%和72%)明显高于卵巢上皮交界性肿瘤(60%、20%和40%)和卵巢上皮良性肿瘤(42%、0%和17%),且有转移患者肿瘤组织中的阳性表达率和异常表达率明显高于无转移者(P<0.05)。A2780、SKOV-3和HO-8910细胞中GMⅡ荧光表达强度逐渐增强,E-cadherin和α-catenin荧光表达强度逐渐减弱。在A2780、SKOV-3、HO-8910中,GMⅡmRNA和蛋白表达水平逐渐增加,而E-cadherin和α-catenin mRNA及蛋白表达水平则逐渐减弱,各组间差异有统计学意义(P<0.05)。结论:GMⅡ、E-cadherin和α-catenin可能在卵巢癌的恶性进展中发挥重要作用,GMⅡ有望成为卵巢癌预后的标志和治疗靶点。Objective： To detect the expressions of GMⅡ （Golgi α-mannosidaseⅡ）, E-cadherin and α-catenin in ovarian epithelial tumor tissues and ovarian cancer cells with different abilities of metastasis. Methods： The expressions of GMⅡ, E-cadherin and α-catenin proteins in ovarian epithelial malignant tumor tissues from 46 cases （including 27 cases of omentum metastasis or lymph node metastasis）, ovarian epithelial borderline tumor tissues from 15 cases, and ovarian epithelial benign tumor tissues from 12 cases were detected by immunohistochemistry. The expression levels of GMⅡ, E-cadherin and α-catenin mRNAs and proteins in A2780, SKOV-3 and HO-8910 cells with different abilities of metastasis were detected by RT-PCR, immunofluorescence and Western blotting, respectively. Results： The positive rate of GMⅡ （87%） and the abnormal expression rates of E-cadherin （80%） and α-catenin （72%） in ovarian epithelial malignant tumor tissues were significantly higher than those in ovarian epithelial borderline tumor tissues （60%, 20% and 40%） and epithelial benign tumor tissues （42%, 0% and 17%）, which were also higher in tumor tissues with omentum metastasis or lymph node metastasis than those without omentum metastasis or lymph node metastasis （P 〈 0.05）. The fluorescence density of GMⅡ was gradually increased in A2780, SKOV-3 and HO-8910 cells, whereas the fluorescence density of E-cadherin and α-catenin was gradually decreased. The expression levels of GMⅡ mRNAs and proteins was gradually increased in A2780, SKOV-3 and HO-8910 cells, whereas the expression levels of E-cadherin and α-catenin mRNAs and proteins were gradually decreased, and the difference were statistically significant among the three cell lines （P 〈 0.05）. Conclusion： GMⅡ, E-cadherin, and α-catenin may play an important role in malignant progression of ovarian tumors, and GMⅡ may become a predictor of prognosis and a therapeutic target for ovarian tumors.

关 键 词：卵巢肿瘤 肿瘤转移 基因表达 GM Ⅱ E—cadherin α—catenin 

分 类 号：R737.31[医药卫生—肿瘤]