NF-κB在幽门螺杆菌阳性和阴性胃癌中的差异表达及临床意义  

Differential Expression of NF-κB in Human Gastric Cancer with Helicobacter pylori Infection and Its Clinical Significance

作　　者：冯水土[1] 陈玉强 陈毅德[1] 王勇军 米彦军 

机构地区：[1]福建医科大学教学医院厦门市第二医院肿瘤科,福建省厦门市361026 [2]福建省厦门市一七四医院肿瘤治疗中心

出　　处：《中国肿瘤临床》2012年第21期1626-1629,共4页Chinese Journal of Clinical Oncology

摘　　要：目的:通过探索NF-κB在胃癌标本中的表达情况,并结合HP感染状态与临床病理资料进行统计学分析,探讨NF-κB与HP感染状态对胃癌的发生、发展的意义。方法:采用免疫组织化学的方法检测随机选取的70例胃癌石蜡标本及56例相应癌旁正常组织标本中NF-κB蛋白的表达情况;Q-PCR方法检测8例活体标本(胃癌与相应癌旁正常组织,其中4例HP^+、4例HP^-)中NF-κB mRNA的表达水平,通过Western blot分析8例活体标本中NF-κB蛋白的表达情况。结果:NF-κB蛋白在HP^+胃癌和HP^-癌旁组织中的表达率为82.50%和26.32%(χ~2=24.867,P<0.05);NF-κB蛋白在HP^+和HP^-胃癌组织中的表达率为82.50%和53.33%(χ~2=6.94,P<0.05) NF-κB蛋白在胃癌组织中表达阳性率为70.00%(49/70),与胃癌的转移、浸润相关,与病理学分级、肿瘤大小无关。活体标本中,HP^+胃癌组织中NF-κB的mRNA和蛋白质表达水平明显高于相应的非癌组织,而在HP^-胃癌组织中的表达水平与相应的非癌组织没有差异。结论:NF-κB蛋白在HP^+胃癌中高表达,而在HP^-胃癌、HP^+和HP^-的癌旁组织中均为低表达NF-κB蛋白的表达与HP^+胃癌发展具有相关性。Objective： To investigate the expression of nuclear factor kappa-light-chain-enhancer of activated B cell （NF-KB） and Helicobacter pylori （HP） infection status with the use of gastric cancer samples and statistical analysis of HP infection status and clinical data, as well as to provide clinical diagnosis and determine gastric cancer treatment procedures. Methods： T total of 70 paraffin-embedded samples of gastric cancer and 56 normal tissues from surgically resected tissues were randomly selected. NF-κB protein expression in the samples was detected by immunohistochemical staining. A total of 8 living specimens （gastric carcinoma and corresponding normal tissues, 4 pairs of HP＋, and 4 pairs of HP＋ were also selected. The mRNA and of NF-κB protein expression in the samples was detected by quality polymerase chain reaction and western blot. Results： The positive rates of NF-κB protein expression in HP＋ gastric carcinoma tissues and adjacent nonneoplastic tissues were 82.50% and 26.32%, respectively. A significant difference in the expression levels was indicated （X2=24.87, P〈0.05, respectively）. The positive rates of NF-κB protein expression in HP＋ and HP- gastric carcinoma tissues were 82.50% and 53.33% respectively. These results indicated a significant difference in the expression levels （~=6.94, P〈0.05, respectively）. The NF-κB protein expression in gastric carcinoma tissues was 70.00% （49/70）. A significant difference in the expression levels was indicated （P〈0.05）. It correlated with tumor metastasis and invasion but not with histopathologic grading and tumor size. Compared with the adjacent nonneoplastic tissues, HP＋ gastric cancer tissues exhibited higher mRNA and NF-κB protein expression in living specimens. However, no significant difference was indicated between HP＋ gastric carcinoma tissues with the adjacent nonneoplastic tissues. Conclusion： NF-κB protein expression was upregulated in the HP＋ gastric carcinoma sample but not in the HP- gastr

关 键 词：胃癌 NF-ΚB 幽门螺杆菌 表达 

分 类 号：R735.2[医药卫生—肿瘤]